目的 建立高效液相色譜法測(cè)定還陽參藥材中綠原酸的方法。方法 采用Sino Chrom DDS-BP色譜柱（250 mm×4.6 mm，5 μm），流動(dòng)相為乙腈-0.4%磷酸水溶液（13∶87），體積流量1.0 mL/min，檢測(cè)波長(zhǎng)327 nm，柱溫25 ℃。結(jié)果 綠原酸進(jìn)樣量在0.046～0.644 μg與峰面積呈良好的線性關(guān)系（r＝1），樣品平均加樣回收率為101.9%，RSD值為1.22%（n＝6）；檢出限和定量限分別為0.01 ng和0.04 ng。結(jié)論 此方法簡(jiǎn)便、準(zhǔn)確、重復(fù)性好，可用于還陽參藥材的質(zhì)量控制。

Objective To establish a method of HPLC for the determination of chlorogenic acid in the herbs of Crepis turczaninowii. Methods The samples were separated on a Sino Chrom DDS-BP column (250 mm × 4.6 mm, 5 μm) with the mobile phase of acetonitrile-0.4% phosphoric acid (13:87). The flow rate was 1.0 mL/min; The detection wavelength was set at 327 nm; And column temperature was 25 ℃. Results There was a good linear relationship between the chlorogenic acid and peak area in the range of 0.046—0.644 μg (r = 1). The average recovery rate was 101.9%, and RSD was 1.22% (n = 6). Conclusion The method is simple, accurate, and highly reproductive, and could be used for the quality control of the herbs of C. turczaninowii.

山西省中藥材、中藥飲片地方標(biāo)準(zhǔn)研究項(xiàng)目（2011024A）