目的 優(yōu)選烏藥總生物堿（TALR）的提取工藝并研究其鎮(zhèn)痛作用。方法 采取乙醇回流法提取，考察乙醇濃度、乙醇用量、提取時(shí)間、提取次數(shù)影響，進(jìn)行L₉(3⁴) 正交試驗(yàn)設(shè)計(jì)，以烏藥總生物堿的紫外吸收峰最大吸光度A值為指標(biāo)進(jìn)行優(yōu)選。鎮(zhèn)痛實(shí)驗(yàn)取最佳提取方法所得TALR的不同濃度水溶液ig給予小鼠后，采用醋酸小鼠扭體法研究TALR的鎮(zhèn)痛作用。結(jié)果 優(yōu)選工藝為：加入14倍量95%乙醇提取1 h，酸溶堿沉得TALR，因其A值遠(yuǎn)高于其他工藝，故判斷此法所得TALR質(zhì)量分?jǐn)?shù)相對最高。藥理實(shí)驗(yàn)表明TALR可顯著減少小鼠扭體次數(shù)。結(jié)論 優(yōu)選工藝簡單可行，質(zhì)量分?jǐn)?shù)較高；TALR具有較強(qiáng)的鎮(zhèn)痛作用。

Objective To optimize the extraction technology of total alkaloids from Lindera Radix (TALR) and to study the analgesic effect. Methods The extraction technology of TALR was optimized by orthogonal experiment with concentration of ethanol, amount of ethanol, extraction time, and extraction times as factors, and with the maximum absorbance of ultraviolet absorption peak (A) of TALR as index. In analgesic experiments, different concentration of TALR aqueous solution extracted by the optimized method was ig administered to mice, and the analgesic effect of TALR was observed by acetic acid writhing method. Results The optimum technology was 1 h extraction with 14 times amount of 95% alcohol and TALR was got after acid-dissolution and alkali-precipitation. The A value of optimum technology outclassed others, so the content of TALR was higher. Pharmacologic experiments indicated TALR could obviously decrease mouse writhing times. Conclusion The optimum technology is easy to be operated and extracted TALR has higher content. TALR has obvious analgesic effect.