目的 研究伸筋草石油醚、氯仿及正丁醇提取物在DPPH法體外模型中的抗氧化作用及作用時間與清除率的關(guān)系。方法 建立DPPH體系，采用HPLC法測定反應(yīng)體系中DPPH變化，觀察伸筋草各提取物不同時間對DPPH自由基的清除率。結(jié)果 伸筋草各提取物對DPPH的清除均有抑制作用，且呈明顯劑量–效應(yīng)關(guān)系，其清除率隨作用時間增長而增加。石油醚、氯仿及正丁醇提取物對DPPH的IC₅₀分別為0.56、0.31、0.13 g/mL；結(jié)論 伸筋草正丁醇提取物具有較強的清除自由基活性。

Objective To investigate the anti-oxidative activities of petroleum ether, ethyl acetatelayer, and butanol extracts from Lycopodii Herba (ELH) using DPPH? model in vitro, and to study the relationship between action time and clearance rate. Methods The DPPH·system was estabilshed, the change of DPPH in the system was determined by HPLC method, and the clearance rates of DPPH? by different ELH at different time points were observed. Results Anti-oxidative activity of butanol extract was the strongest, IC₅₀ values of petroleum ether, ethyl acetatelayer, and butanol extracts were 0.56, 0.31, and 0.13 g/mL, respectively. Conclusion The butanol extract has a strong ability of free radical scavenging.

國家863課題（2004 AA.2Z3730-13）