目的 建立通光藤藥材及消癌平片中通光藤苷A的HPLC測(cè)定方法。方法 色譜柱為Sinochrom ODS-BP分析柱（250 mm×4.6 mm，5 μm），流動(dòng)相為乙腈-0.1%磷酸（41∶59）；檢測(cè)波長(zhǎng)230 nm。結(jié)果 通光藤苷A在20.2～404.0 μg與峰面積積分值呈良好的線性關(guān)系，r＝0.999 95，平均加樣回收率為96.6%，RSD值為2.19%（n＝6）。結(jié)論 該方法簡(jiǎn)單，結(jié)果準(zhǔn)確，穩(wěn)定性好，重現(xiàn)性高，可用于通光藤藥材及消癌平片定量測(cè)定及質(zhì)量控制。

Objective To develop an HPLC method for determination of tenacissosides A in Marsdenia tenacissima and Xiaoaiping Tablets. Methods Sinochrom ODS-BP column was used (250 mm × 4.6 mm, 5 μm), the mobile phase consisted of acetonitrile-0.1% (41:59), and the UV detection wavelength was at 230 nm. Results Good linear correlation for tenacissosides A was found in the concentration range of 20.2―404.0 μg (r = 0.999 95). The average recovery rate was 96.6%, with RSD of 2.19% (n = 6). Conclusion The method is simple, accurate, and stable which is fit for determination and quality control of Marsdeniae Tenacissimae Caulis and Xiaoaiping Tablets.

通關(guān)藤種質(zhì)鑒定與藥材質(zhì)量相關(guān)性研究（2011011035-1）