目的 探討葒草苷對急性心肌梗死模型大鼠的保護作用及機制。方法 建立異丙腎上腺素致大鼠急性心肌梗死模型，將大鼠分為正常對照組、模型組、陽性藥普萘洛爾組及低、高劑量葒草苷干預(yù)組（1.0、2.0 mg/kg）；采用多道生理記錄儀采集心電圖、血流動力學(xué)指標，檢測血清中心肌酶、抗氧化物酶的活性，HE染色觀察心肌組織病理學(xué)改變，ELISA法檢測心肌組織環(huán)鳥苷酸（cGMP）水平。結(jié)果 與模型組比較，預(yù)防性ip 2.0 mg/kg葒草苷連續(xù)7 d，可降低大鼠心電圖ST段高度變化，明顯改善大鼠血流動力學(xué)指標，降低大鼠血清心肌酶LDH、ALT和AST的水平，減輕心肌梗死大鼠心肌纖維變性壞死及水腫程度，亦可升高大鼠血清中抗氧化酶SOD、GSH-Px活性及心肌組織中cGMP水平。結(jié)論 葒草苷對大鼠急性心肌梗死具有明顯保護作用，其保護機制可能與增強體內(nèi)抗氧化酶活性，提高心肌組織cGMP水平和調(diào)節(jié)NO/cGMP信號通路有關(guān)。

Objective To investigate the protective effect of orientin (Ori) on acute myocardial infarction (AMI) in rats and study its mechanism. Methods The rat model with AMI was induced by sc injection of isoproterenol. The rats were randomly divided into five groups, such as control, model, Propranolol (positive control), low- and high-dose Ori (1.0 and 2.0 mg/kg) groups. The electrocardiogram (ECG) and hemodynamic parameters were recorded by PowerLab, the activities of cardiac enzymes and anti- oxidant enzymes were determined, histopathological changes of myocardial tissue were measured by HE staining, and the level of cyclic guanosine monophosphate (cGMP) was detected by ELISA. Results Ip injection of Ori (2.0 mg/kg) for 7 d could reduce the ST segment height of ECG, improve the hemodynamic parameters in rats obviously, decrease the serum myocardial enzyme levels, reduce the myocardial fiber degeneration, necrosis, and edema, and increase the superoxide dismutase and glutathione peroxidase activities and the myocardial cGMP level. Conclusion Ori could protect rats against AMI induced by isoproterenol, which may contribute to enhancing the anti-oxidase activities, elevating the cGMP level of myocardial tissue, and regulating the NO/cGMP signal pathway.

國家自然科學(xué)基金資助項目（81173656）；國家民族事務(wù)委員會資助項目（10ZY08）