目的 從免疫學(xué)角度探討大豆多糖對荷瘤小鼠抗腫瘤作用的機制。方法 采用分光光度計測定大豆多糖對S₁₈₀荷瘤小鼠巨噬細(xì)胞一氧化氮生成量的影響，利用酶標(biāo)儀測定大豆多糖對S₁₈₀荷瘤小鼠腹腔巨噬細(xì)胞吞噬功能的影響，定量溶血分光光度法檢測B細(xì)胞功能。結(jié)果 與對照組比較，大豆多糖各組均能顯著提高小鼠巨噬細(xì)胞的吞噬活性，同時能明顯提高荷瘤小鼠巨噬細(xì)胞產(chǎn)生一氧化氮的能力，并能使體內(nèi)B淋巴細(xì)胞數(shù)量增加而提高抗體生成量。結(jié)論 大豆多糖通過調(diào)節(jié)荷瘤小鼠免疫功能發(fā)揮抗腫瘤作用。

Objective To study the antitumor effects of soybean polysaccharide (SPS) on S₁₈₀^- bearing mice and the mechanism from the point of immunological view. Methods The effect of SPS on the nitric oxide (NO) generation by macrophage in S₁₈₀^- bearing mice was detected by spectrophotometer; ELIASA was used to determine the effect of SPS on phagocytosis of macrophage; The function of B-lymphocytes was detected by quantitative haemolysis specteophotomeric method. Results Compared with the control group, SPS could significantly enhance the phagocytosis of macrophage and increase the NO generation of macrophage as well as the content of B-lymphocytes in S₁₈₀^- bearing mice, so as to increase the generation of antibodies. Conclusion SPS could play its antitumor role by regulating the immune function of mice.

黑龍江省教育廳項目（12521133）