目的 研究庫拉索蘆薈多糖的保濕特性、經(jīng)皮吸收特性以及皮膚安全性。方法 采用超聲輔助提取蘆薈活性多糖，并通過人體皮膚水分含量和水分散失量測試法對所制備的蘆薈活性多糖的保濕特性（包括鎖水、保水兩個(gè)方面）進(jìn)行評價(jià)；Franz擴(kuò)散池體外透皮吸收法評價(jià)其透皮吸收特性；人體斑貼試驗(yàn)評價(jià)其皮膚安全性。結(jié)果 與空白對照組比較，庫拉索蘆薈多糖可顯著提高皮膚水合狀態(tài)（P＜0.05），同時(shí)降低經(jīng)皮失水（P＜0.05）；多糖質(zhì)量濃度為5%時(shí)，皮膚滲透速率達(dá)到0.251 0 mg/(h?cm²)，24 h累計(jì)滲透量達(dá)（4.151 9±0.046 2）mg/cm²；人體皮膚斑貼試驗(yàn)中全部評定為0級反應(yīng)。結(jié)論 庫拉索蘆薈活性多糖對皮膚具有顯著的保濕功效以及較強(qiáng)的皮膚滲透力，并且高度安全，可作為優(yōu)良的保濕添加劑在化妝品等膚用產(chǎn)品中廣泛應(yīng)用。

Objective To investigate the moisturizing properties, percutaneous absorption, and skin safety of active polysaccharide from Aloe vera (APAV). Methods The APAV was extracted from Aloe vera by the ultrasonic assisted extraction. The moisturizing property of the prepared sample was evaluated by the human skin moisture value determination and transepidermal water loss determination. The transdermal absorption characteristics of APAV was investigated by Franz diffusion cell in vitro transdermal absorption method. The skin safety was investigated by the human skin patch test method. Results APAV could significantly improve skin hydration status (P < 0.05), as well as reduce the skin water loss (P < 0.05). When the mass concentration of APAV was 5%, the skin penetration rate was 0.251 0 mg/(h?cm²) and the quantity of the transdermal absorption was (4.151 9 ± 0.046 2) mg/cm² in 24 h. The results of the human skin patch test were all in 0 level. Conclusion The results show that APAV has the significant moisturizing effect and strong penetration of the skin and high security on the human skin. It could be used as the moisturizing additive in cosmetic skin products.

十二五科技支撐計(jì)劃（2012BAD36B02）；北京市優(yōu)秀人才培養(yǎng)資助計(jì)劃（2012D005003000008）；北京市屬高等學(xué)?？茖W(xué)技術(shù)與研究生教育創(chuàng)新工程建設(shè)項(xiàng)目（PXM2012_014213000068）