目的 建立不同粒徑連翹粉體的高效液相色譜法（HPLC）特征指紋圖譜，并對(duì)其進(jìn)行系統(tǒng)聚類分析，探討粒徑與化學(xué)成分溶出特征的相關(guān)性。方法 將連翹藥材進(jìn)行粉碎、過(guò)篩，按粒徑大小分成10組，采用HPLC法建立不同粒徑連翹粉體的指紋圖譜，結(jié)合主要共有指紋峰信息，對(duì)不同粒徑連翹粉體進(jìn)行系統(tǒng)聚類分析。結(jié)果 不同粒徑連翹粉體共有8個(gè)HPLC特征峰，根據(jù)各粉體單位質(zhì)量峰面積，不同粒徑連翹粉體被分為不同層次的3類，其中超微粉單位質(zhì)量峰面積最大。結(jié)論 不同粒徑連翹粉體與化學(xué)成分溶出具有良好的相關(guān)性，超微粉碎技術(shù)可以促進(jìn)有效成分的溶出，在粉碎加工藥材方面具有很好的應(yīng)用前景。

Objective To establish the chromatographic fingerprint of Forsythiae Fructus powder (FFP) with different particle diameters and to explore the correlation between the particle diameter and the dissolution characteristic of chemical constituents by hierarchical cluster analysis. Methods Forsythiae Fructus was crushed, filtered, and divided into ten groups according to particle diameter. The chromatographic fingerprint of FFP with different particle diameters was established by HPLC. FFP with different particle diameters was analyzed by hierarchical cluster analysis. Results The eight characteristic peaks were identified in FFP with different particle diameters. And they could be divided into three categories according to peak area per unit mass. The biggest peak area per unit mass was supper micropowder. Conclusion There are good correlations between particle diameter and dissolution characteristic of chemical constituents. Superfine pulverizing technology could be used to promote the dissolution rate of chemical constituents and have a good practical future in the crushing process of crude drugs.

湖南省科技廳重點(diǎn)項(xiàng)目（06FJ2005）