目的 篩選適用于分離純化蓮子心生物堿的大孔吸附樹(shù)脂。方法 考察吸附性能較好的D101、AB-8、DA201 3種大孔吸附樹(shù)脂對(duì)蓮心堿的吸附能力及洗脫參數(shù)，并用HPLC定量分析了蓮心堿的量。結(jié)果 D101樹(shù)脂對(duì)蓮子心中生物堿有較好的吸附分離效果。先用水洗除雜，再用6倍樹(shù)脂體積、乙醇體積分?jǐn)?shù)為80%、pH 3的洗脫劑洗脫，濃縮干燥，產(chǎn)品中蓮心堿的質(zhì)量分?jǐn)?shù)為10.2%、產(chǎn)品收率為24.6%。結(jié)論 該工藝簡(jiǎn)單可行，分離效果好，適合蓮子心生物堿的分離純化。

Objective To screen the macroporous resin for the separation and purification of alkaloids from Nelumbinis Plumula. Methods The resins such as D101, AB-8, and DA201 were studied for their adsorption capability and elution parameters. The contents of liensinine were determined by HPLC. Results D101 resin had a preferable adsorptive and separative effect for the alkaloids in Nelumbinis Plumula. The preferable desorption ratio could be obtained by using 80% ethanol (pH 3) as eluting solvent and 6 BV. Under these conditions, the content of alkaloids in Nelumbinis Plumula was 10.8% and the yield was 2.6%. Conclusion The method is easy and possesses the popularization potential and good efficacy. It could be used for the separation and purification of alkaloids in Nelumbinis Plumula.

湖南省教育廳基金研究項(xiàng)目（11C1256）