目的 建立毛支清口服液中tortoside A的定量測(cè)定方法。方法 采用高效液相色譜（HPLC）法，選用Venusil MP C₁₈分析柱（250 mm×4.6 mm，5 μm），乙腈-水（18∶82）為流動(dòng)相，檢測(cè)波長(zhǎng)為207 nm，體積流量為1.0 mL/min，柱溫30 ℃。結(jié)果 Tortoside A進(jìn)樣質(zhì)量濃度為1.76～35.20 μg/mL與峰面積呈良好的線性關(guān)系，回歸方程Y＝83 335X－47 442，r＝0.999 7，平均回收率為101.53%，RSD值為2.50%。結(jié)論 所建立的HPLC法簡(jiǎn)便、準(zhǔn)確、靈敏度高、重復(fù)性好，可控制毛支清口服液的質(zhì)量。

Objective To establish a method for the determination of tortoside A in Maozhiqing Oral Liquid (MOL). Methods The tortoside A was determined by high performance liquid chromatography (HPLC). The Venusil MP C₁₈ column (4.6 mm × 250 mm, 5 μm) was used, the mobile phase was acetonitrile-water (18:82), the detection wavelength was 207 nm, the flow rate was 1.0 mL/min, and the column temperature was 30 ℃. Results Tortoside A has a good linear relationship with the peak area within the range of 1.76—88.00 μg/mL with an regression equation Y = 83 335X－47 442 (r = 0.999 7). The average recovery was 101.53%, and RSD was 2.50%. Conclusion The established method of HPLC could be used for the quality control of MOL for its simplicity, accuracy, high sensitivity, and good reproducibility.

國(guó)家自然科學(xué)基金資助項(xiàng)目（81073151）