目的 通過給予大鼠伸筋草提取物，探討其中主要活性成分玉柏堿的藥動學(xué)特征。方法 利用RP-HPLC法，以Centurysil C₁₈ EPS為色譜柱，甲醇-水（61∶39）為流動相；紫外檢測器測定大鼠ig伸筋草提取物后血漿中玉柏堿的血藥濃度，計算藥動學(xué)參數(shù)。結(jié)果 玉柏堿在大鼠體內(nèi)呈二室模型分布，線性范圍為0.354～14.16 μg/mL，r＝0.999 8。日內(nèi)和日間精密度均小于5%。玉柏堿口服給藥后在大鼠體內(nèi)的主要藥動學(xué)參數(shù)為：t_1/2為2.681 h，t_max為0.75 h，C_max為6.309 mg/L，AUC_(0→t) 為16.626 mg/L，AUC_(0→∞) 為18.798 mg/L。結(jié)論 本方法適用于大鼠血漿中玉柏堿的檢測及其體內(nèi)藥動學(xué)研究。

Objective To study the in vivo pharmacokinetic characteristics of α-obscurine in rats after the ig administration of Lycopodium japonicum extract (LJE). Methods The RP-HPLC was used to monitor the plasma concentration of α-obscurine in rats after the ig administration of LJE. Methanol-water (61:39) as the mobile phase on Centurysil C₁₈ EPS column. The pharmacokinetic parameters were estimated by the DAS2.0 computer program. Results The concentration-time profiles were fitted with an open two compartment model system. The main pharmacokinetic parameters were t_1/2 2.681 h, t_max 0.75 h, C_max 6.309 mg/L, AUC_(0→t) 16.626 mg/L, AUC_{( 0→∞)} 18.798 mg/L. Conclusion The HPLC method is appropriate for the determination and pharmacokinetic study on α-obscurine in rat plasma after the ig administration of LJE.

國家863課題（2004 AA.2Z3730-13）