目的 探討不同劑型的垂盆草對(duì)大鼠四氯化碳（CCl₄）急性肝損傷模型的保護(hù)作用。方法 Wistar大鼠80只，隨機(jī)分成正常組、模型組、鮮垂盆草榨汁高、低劑量組及鮮垂盆草煎煮組、干垂盆草煎煮組、垂盆草顆粒組、垂盆草配方顆粒組。ig給藥，1 次/d，連續(xù)7 d。第7天，除正常組外，給予大鼠ip 50% CCl₄橄欖油造模。觀察大鼠的一般情況，檢測(cè)肝功能指標(biāo)總膽紅素（Tbil）、丙氨酸氨基轉(zhuǎn)移酶（ALT）、門冬氨酸氨基轉(zhuǎn)移酶（AST）及肝組織丙二醛（MDA）、超氧化物歧化酶（SOD）、一氧化氮（NO）的含量和肝臟組織學(xué)變化情況。結(jié)果 干垂盆草煎煮組可顯著降低大鼠肝質(zhì)量。干垂盆草煎煮組、垂盆草顆粒組、垂盆草配方顆粒組均能降低大鼠血清ALT水平，干垂盆草煎煮組能降低大鼠血清AST水平。干垂盆草煎煮組、垂盆草顆粒組和垂盆草配方顆粒組均可降低大鼠肝組織MDA、NO的含量，提高大鼠肝組織SOD活力。干垂盆草煎煮組、垂盆草顆粒組、垂盆草配方顆粒組顯著改善肝小葉細(xì)胞的變性和炎癥壞死，減輕匯管區(qū)的炎細(xì)胞浸潤。結(jié)論 干垂盆草煎煮液、垂盆草顆粒、垂盆草配方顆?？杀Ｗo(hù)CCl₄導(dǎo)致的大鼠急性肝損傷，且起到抗脂質(zhì)過氧化的作用。

Objective To explore the preventive effect of different preparations of Sudum sarmentosum on the model of carbon tetrachloride (CCl₄)-induced acute liver injury in rats. Methods Purebred 80 Wistar rats were randomly divided into normal, model, the high- and low-dose fresh sarmentosum juice groups, the decoction of fresh sarmentosum, the decoction of sarmentosum, the sarmentosum granules, and the sarmentosum formula granules groups. Gavage started from the first day of the experiment, once a day, for consecutive 7 d. All rats in each group accepted ip injection of 50% CCl₄ olive oil except the normal group on the day 7. General changes of vital signs, the liver function (Tbil, ALT, and AST), levels of MDA, SOD, and NO in liver tissue, and liver histology examination were observed. Results The dry sarmentosum decoction group could significantly reduce the weight of liver. The dry sarmentosum decoction group, the sarmentosum granules group and the sarmentosum formula granules group could reduce the levels of ALT in serum of rats. The dry sarmentosum decoction group could also reduce the levels of AST in serum of rats. The dry sarmentosum decoction group, the sarmentosum granules group, and the sarmentosum formula granules group could reduce the levels of MDA and NO and improve the levels of SOD in liver tissue of rats. The dry sarmentosum decoction group, the sarmentosum granules group and the sarmentosum formula granules group could significantly improve the hepatic lobule cell degeneration inflammation and necrosis, reduce the infiltration of inflammatory cell in portal areas. Conclusion The dry sarmentosum decoction solution, the sarmentosum granules, and the sarmentosum formula granules have a protective effect on rats with CCl₄-induced acute liver injury and show the anti-lipid peroxidation.

國家中醫(yī)藥管理局重點(diǎn)學(xué)科建設(shè)—中醫(yī)傳染病學(xué)