目的 觀察雙黃花顆粒的鎮(zhèn)咳、祛痰及調(diào)節(jié)免疫作用。方法 通過(guò)小鼠氨水引咳和豚鼠枸櫞酸引咳試驗(yàn)，觀察雙黃花的鎮(zhèn)咳作用；通過(guò)小鼠酚紅排泄試驗(yàn)觀察雙黃花的祛痰作用；通過(guò)小鼠碳粒廓清試驗(yàn)，觀察雙黃花的調(diào)節(jié)免疫作用。結(jié)果 在小鼠氨水引咳試驗(yàn)中，雙黃花高、中劑量組能顯著延長(zhǎng)濃氨水致咳的潛伏期，差異具有統(tǒng)計(jì)學(xué)意義（P＜0.05）；雙黃花高劑量組能顯著減少小鼠濃氨水致咳的次數(shù)，差異具有統(tǒng)計(jì)學(xué)意義（P＜0.05）；在豚鼠枸櫞酸引咳試驗(yàn)中，雙黃花高劑量組能顯著延長(zhǎng)豚鼠枸櫞酸引咳的潛伏期和減少咳嗽次數(shù)，差異具有統(tǒng)計(jì)學(xué)意義（P＜0.01）；在小鼠酚紅排泄試驗(yàn)中，雙黃花高劑量組能顯著增加氣管酚紅排泄量，差異具有統(tǒng)計(jì)學(xué)意義（P＜0.05）；在小鼠碳粒廓清試驗(yàn)中，雙黃花高、中劑量組顯著提高小鼠廓清指數(shù)和吞噬指數(shù)，差異具有統(tǒng)計(jì)學(xué)意義（P＜0.05、0.01）。結(jié)論 雙黃花顆粒具有較好的鎮(zhèn)咳、祛痰及調(diào)節(jié)免疫作用。

Objective To evaluate the relieving cough, expelling phlegm, and immunomodulation of Shuanghuanghua Granule (SG). Methods Cough was induced by ammonia in mice and citric acid in guinea pig. The number of cough was recorded to indicate the effect of SG. The expectorant activities were detected by testing the amount of phlegm secreted in mice. Immunomodulation was evaluated by testing the expectorant carbon clearance in mice. Results Cough was induced by ammonia in mice, high- and mid-dose SG groups could apparently prolong the coughing incubation period (P < 0.05) and high-dose SG group could apparently reduce coughing frequency (P < 0.05); Cough was induced by citric acid in guinea pig, high-dose SG group could apparently increase phenol red excretion in mice (P < 0.05); In the expectorant activities experimental, high-dose SG group could apparently increase phenol red sputem excretion in mice (P < 0.05); In the expectorant carbon clearance in mice, high- and mid-dose SG groups could apparently decrease the expurgatory index and phagocytic index (P < 0.01, 0.05). Conclusion SG has the significant effects of relieving cough, expelling phlegm, and immunomodulation.

湖南省教育廳科研項(xiàng)目（11B090）；湖南省中醫(yī)藥管理局科研項(xiàng)目（2009030）