目的 探究一種有效分離純化菊米黃酮的大孔樹脂并對純化工藝條件進(jìn)行優(yōu)化。方法 以樹脂的飽和吸附容量、吸附速率和解吸附率為參考指標(biāo)，從10種不同的大孔吸附樹脂中篩選出綜合性能較佳的樹脂，并進(jìn)一步考察此樹脂純化菊米總黃酮的影響因素和分離純化效果。結(jié)果 HPD-100樹脂的綜合性能較好，飽和吸附容量達(dá)到60.2 mg/g（以濕重計(jì)），且吸附迅速，解吸附完全。較理想的吸附工藝：在樹脂徑高比為1∶10的條件下，質(zhì)量濃度為4.3 mg/mL供試液以15 mL/min上柱時(shí)，最佳上樣量為5倍柱床體積（BV），靜態(tài)吸附時(shí)間為2 h；較理想的洗脫工藝：先用6 BV的純水洗雜質(zhì)，再以60%的乙醇為洗脫劑，洗脫體積流量為8 mL/min時(shí)，用量為3 BV，所得菊米總黃酮質(zhì)量分?jǐn)?shù)為（81.6±0.9）%。結(jié)論 HPD-100是一種分離純化菊米黃酮較佳的樹脂，其工藝簡單，具有較好的工業(yè)化生產(chǎn)前景。

Objective To screen a macroporous resin for effective separating and purifying the total flavonoids from the buds of Chrysanthemum indicum (TFBCI) and to further optimize the process conditions. Methods Through critical evaluating the absorption and desorption properties of macroporous resins including the absorption capacity, absorption velocity, and desorption rate, a preferable resin was selected from 10 kinds of macroporous resins, and the influence factors and purification effect on TFBCI were investigated further. Results HPD-100 resin had outstanding comprehensive performance, maximum static adsorption capacity was 60.2 mg/g (wet resin), and the adsorption was rapid and desorption complete. The optimum adsorption process was: under the condition of the resin’s diameter height ratio of 1:10, the sample solution concentration 4.3 mg/mL and flow rate 7 mL/min, the best processing volume was 5 BV, and static adsorption time was 2 h; The optimum elution process was: wash the impurities with 6 BV of pure water, elute product with 60% ethanol at the speed of 8 mL/min. The purity of the final product reached to (81.6 ± 0.9)%. Conclusion HPD-100 is an ideal resin for separating and purifying the TFBCI, and its process is simple and has preferable industrialization prospect.

國家自然科學(xué)基金資助項(xiàng)目（21076191，21006097，21276237）