目的 研究凌霄花提取物抗凝血作用，確定凌霄花抗凝血活性部位。方法 通過(guò)血漿復(fù)鈣實(shí)驗(yàn)測(cè)定小鼠血漿復(fù)鈣時(shí)間（PRT）和斷尾法測(cè)定小鼠出血時(shí)間（BT）實(shí)驗(yàn)，比較凌霄花不同提取物的藥效活性。結(jié)果 凌霄花水提物、醇提物及水提醇沉上清液均具有抗凝血活性；醇提物萃取后正丁醇層藥效最顯著；水提醇沉上清液過(guò)大孔樹(shù)脂后10%乙醇部位藥效最顯著。結(jié)論 凌霄花抗凝血活性主要存在于極性較大部位。

Objective To investigate the anti-coagulation of the extracts from the flowers of Campsis gradiflora (CG) and to determine the active fraction. Methods Plasma recalcification time (PRT) of mice was evaluated in plasma recalcification experiment and bleeding time (BT) was determined by cutting-tail method. The anti-coagulation activities of different fractions in the extracts from CG were compared. Results The water extract, 80% ethanol extract, and the extract by water-extraction and alcohol-precipitation from the flowers of CG all have the anti-coagulation activities. The butanol layer from 80% ethanol extract and the macroporous resin eluate of 10% ethanol from the extract by water-extraction and alcohol-precipitation showed the significant anti-coagulation. Conclusion The more polar fractions of the extracts from the flowers of CG are the main effective fractions

上海市衛(wèi)生局中醫(yī)藥科研基金資助項(xiàng)目（WSJ1219）