目的 探討杠柳毒苷在體外對(duì)人乳腺癌MDA-MB-468細(xì)胞和人肝癌HepG2細(xì)胞增殖的影響。方法 MTT法觀察杠柳毒苷對(duì)人乳腺癌MDA-MB-468細(xì)胞和人肝癌HepG2細(xì)胞增殖的抑制作用，流式細(xì)胞術(shù)觀察杠柳毒苷對(duì)兩種腫瘤細(xì)胞的細(xì)胞增殖周期作用。結(jié)果 與對(duì)照組比較，杠柳毒苷能明顯抑制兩種腫瘤細(xì)胞的增殖，其抑制率與藥物濃度和作用時(shí)間呈正相關(guān)。流式細(xì)胞儀檢測(cè)發(fā)現(xiàn)，杠柳毒苷對(duì)乳腺癌MDA-MB-468細(xì)胞和肝癌HepG2細(xì)胞持續(xù)作用24 h后，可以使G₀/G₁期細(xì)胞增多，G₂/M期細(xì)胞減少。結(jié)論 杠柳毒苷具有抑制乳腺癌MDA-MB-468細(xì)胞和肝癌HepG2細(xì)胞增殖的作用，并可將乳腺癌MDA-MB-468細(xì)胞和肝癌HepG2細(xì)胞的細(xì)胞生長(zhǎng)周期阻滯在G₀/G₁期。

Objective To discuss the effect of periplocin on the proliferation of the breast carcinoma MDA-MB-468 cells and hepatocellular carcinoma HepG2 cells in vitro. Methods MTT method was used to examine the proliferation of MDA-MB-468 and HepG2 cells, and flow cytometry was used to observe the effect of periplocin on cell cycle of the two kinds of tumer cells. Results Compared with the control group, periplocin could obviously inhibit the proliferation of two kinds of cells, and the inhibitory rate was positively correlated with drug level and action time. By flow cytometry, it was found that after 24 h treatment, periplocin induced cell cycle (G₀/G₁) arrest in MDA-MB-468 and HepG2 cells . Conclusion Periplocin could inhibit the proliferation of the breast carcinoma MDA-MB-468 and hepatocellular carcinoma HepG2 cells, and block their cell cycle in G₀/G₁ phase.

天津市衛(wèi)生局課題（2005075）