目的 建立消毒飲優(yōu)化方膠囊中異嗪皮啶的HPLC測定方法。方法 采用phenomenex Synergi 4 u Fusion-RP 80A柱（250 mm×4.60 mm，4 μm），流動相為乙腈-1%磷酸（20∶80），體積流量為1.0 mL/min，柱溫為40 ℃，檢測波長為342 nm。結(jié)果 異嗪皮啶進樣量在0.100 0～1.000 0 μg與峰面積呈良好的線性關(guān)系（r＝0.999 9），平均回收率為95.04%，RSD＝1.84%（n＝6）。結(jié)論 該方法操作簡便、準確、專屬性強、重復(fù)性好，可用于消毒飲優(yōu)化方膠囊中異嗪皮啶的含量測定。

Objective To establish a method for the content determination of isofraxidin in Optimizing Xiaoduyin Capsules. Methods The HPLC was performed on the phenomenex Synergi 4 u Fusion-RP 80A column (250 mm × 4.60 mm, 4 μm) with the mobile phase of acetonitrile-1% phosphoric acid (20:80); The flow rate was 1.0 mL/min, the column temperature was 40 ℃, and the detection wavelength 342 nm. Results The sample size of isofraxidin during the range of 0.100 0—1.000 0 μg showed a good linear relation with the peak area (r = 0.999 9), and the average recovery rate was 95.04%, with RSD of 1.84% (n = 6). Conclusion The method is simple, reliable, and specific, with good reproducibility, and could be used to determine the content of isofraxid in Optimizing Xiaoduyin Capsules.

廣西科學(xué)研究與技術(shù)開發(fā)計劃課題（桂科攻11107009-2-4）；廣西壯族自治區(qū)中醫(yī)藥科技專項課題（GZYZ1125）；廣西教育廳校地校企共建科技創(chuàng)新平臺項目（桂教科研 [2011] 9號）；廣西教育廳校地校企共建科技創(chuàng)新平臺項目（桂教科研 [2013] 8號-3）