目的 研究冬蟲夏草菌絲體水提醇沉物的體外抗腫瘤活性及其機制。方法 采用熱水浸提-醇沉法得到水提物，高效液相色譜儀測定水提醇沉物中腺苷的量；采用MTT法測定其抗腫瘤活性，利用流式細胞儀結合碘化丙錠染色法檢測其對細胞周期的抑制。結果 實驗表明水提醇沉物能抑制人肝癌HepG2細胞株及大細胞肺癌NCI-H460細胞株的增殖，并呈濃度相關性；半數(shù)抑制濃度（IC₅₀）值分別為（1.49±0.19）和（1.67±0.27）mg/mL。細胞周期分析表明，水提醇沉物分別阻滯HepG2及NCI-H460細胞周期于G₂/M期、S期，并可誘導上述兩種細胞發(fā)生凋亡。結論 冬蟲夏草水提醇沉物通過阻滯HepG2及NCI-H460細胞周期循環(huán)，誘導其凋亡，從而表現(xiàn)出良好的增殖抑制活性。為深入研究冬蟲夏草菌絲體水提醇沉物抗腫瘤的機制提供了實驗證據(jù)。

Objective To study the anti-tumor activity of water extract from artificially propagated Cordyceps sinensis mycelia in vitro and its mechanisms. Methods Water extract from C. sinensis mycelia was obtained using hot-water-extraction combined with alcohol precipitation method and the content of adenosine was determined using HPLC; MTT assay was performed to assess the cell growth inhibition, and the distribution of cell cycle was determined by flow cytometric analysis of propidium iodide staining. Results It was found that the water extract of C. sinensis mycelia could inhibit human hepatoma HepG2 cells and large cell lung cancer NCI-H460 cells growth in a concentration-dependent manner; the IC₅₀ values were (1.49 ± 0.19) and (1.67 ± 0.27) mg/mL, respectively. Cell cycle analysis showed that water extract arrested HepG2 cell cycle at G₂/M phase, and arrested NCI-H460 cell cycle at S phase; Furthermore, the water extract could induce the above two cell lines apoptosis. Conclusion The water extract from C. sinensis mycelia could inhibit HepG2 and NCI-H460 cells growth through cell cycle arrest and apoptosis induction. This study provides the important experimental evidence which would contribute to the further study on the anti-tumor mechanisms of the water extract from C. sinensis mycelia.

國家自然科學基金項目（21161003，21364002）；廣西自然科學基金項目（2013GXNSFGA019001，2012GXNSFDA053007，2011GXNSFA018044，2013GXNSFBA019200）；廣西“新世紀十百千人才工程”、廣西醫(yī)藥人才小高地項目（1105，1313）、廣西壯族自治區(qū)教育廳一般項目（2013YB025）；藥用資源化學與藥物分子工程教育部重點實驗室主任基金、開放基金資助項目（CMEMR2012-A01，CMEMR2012-A12，CMEMR2008-B01，桂科能07109001-19）；廣西師范大學校級重點項目（2011ZD006）