目的 研究平炎舒消膏（PSO）的抗炎作用并初步探討其作用機(jī)制。方法 采用小鼠腹腔毛細(xì)血管通透性模型、大鼠足腫脹模型及大鼠棉球肉芽腫模型，觀察PSO的在體抗炎作用。采用酶聯(lián)免疫吸附法（ELISA）和生物測定法，觀察PSO對脂多糖（LPS）誘導(dǎo)的巨噬細(xì)胞（RAW264.7）釋放的白細(xì)胞介素-1（IL-1β）和腫瘤壞死因子-α（TNF-α）含量及生物學(xué)活性的影響，探索PSO的抗炎作用機(jī)制。結(jié)果 體內(nèi)實(shí)驗(yàn)中，PSO顯著抑制醋酸所致小鼠腹腔毛細(xì)血管通透性升高，明顯降低大鼠自身血所致足跖腫脹，減輕大鼠棉球肉芽腫。體外實(shí)驗(yàn)中，PSO濃度相關(guān)性地抑制LPS誘導(dǎo)的RAW264.7釋放IL-1β及TNF-α，降低IL-1β對胸腺細(xì)胞的增殖效應(yīng)及TNF-α對L-929細(xì)胞的殺傷作用。結(jié)論 平炎舒消膏可減輕各期炎癥形成，其機(jī)制可能與抑制巨噬細(xì)胞釋放炎性因子有關(guān)。

Objective To study the anti-inflammatory effects of Pingyan Shuxiao Ointment (PSO) and its possible mechanism. Methods The anti-inflammatory effects were investigated in the peritoneal capillary permeability model of mice induced by acetic acid, in the paw edema model of rats induced by autologous blood, and granuloma model of rats induced by cotton ball. The anti-inflammatory mechanism of PSO was observed by assessing the content and biological activity of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) on RAW264.7 cells induced by lipopolysaccharide (LPS), using enzyme-linked immunosorbent (ELISA) and biological assay. Results PSO in vivo significantly improved the degree of the peritoneal capillary permeability of mice, decreased autologous blood-induced paw edema, and reduced cotton ball-induced granuloma of rats. PSO in vitro concentration-dependently inhibited LPS-induced RAW264.7 cell releasing IL-1β and TNF-α, reduced IL-1β’s proliferation effect on thymocyte and TNF-α’s capability on killing L929 cell. Conclusion PSO has the obvious anti-inflammatory effect, which may be mediated through the inhibition of the cytokines levels in the inflammatory tissues.