目的 建立超快速液相色譜-質譜/質譜聯用（UFLC-MS/MS）法測定酚酞片中酚酞的量。方法 樣品以乙醇提取、微孔濾膜濾過、離心后，通過電噴霧離子化（ESI），采用多反應檢測（MRM）方式進行正離子檢測，用于定量分析的檢測離子為m/z 319.0→225.2。采用Shim-pack XR-ODS色譜柱（75 mm×3.0 mm，2.0 μm）分離，以乙腈-水-甲酸（55∶45∶0.1）為流動相，體積流量為0.40 mL/min，在3 min內完成酚酞定量分析。結果 線性范圍為2.5～1 000.0 ng/mL，最低檢測限為2.5 ng/mL；日內RSD小于1.85%，日間RSD小于2.56%。結論 本方法靈敏度高，分析速度快，操作簡單，可作為酚酞片中酚酞質量控制方法。

Objective To establish an ultra fast liquid chromatography/tandem mass spectrometric (HPLC-MS/MS) method for the determination of phenolphthalein in Phenolphthalein Tablet (PT). Methods The samples were extracted by ethanol, filtered with microporous membrane, and centrifugated. LC/MS/MS detection was performed by using a triple-stage quadrupole mass spectrometer working in multiple reaction monitoring mode (MRM) with positive electrospray ionization (ESI), using the transitions of m/z 319.0→225.2 of phenolphthalein. Chromatographic separation was achieved using Shim-pack XR-ODS column (75 mm × 3.0 mm, 2.0 μm) with isocratic flow and elution with acetonitrile-water-formic acid (55:45:0.1) and a flow rate of 0.4 mL/min. The analyte was quantified in a single run within 3 min. Results The linearity was demonstrated over the expected concentration range of 2.5— 1 000.0 ng/mL, and the lower limit of quantification (LLOQ) was 2.5 ng/mL. The intra-day precision expressed as RSD was 1.85% or less, while the inter-day result was 2.56% or less for phenolphthalein. Conclusion The method is more convenient with good repeatabilityand high sensitivity, and could be successfully applied for the determination of phenolphthalein in PT.