目的 研究注射用三磷酸腺苷輔酶胰島素（能量合劑）對心肌缺血再灌注損傷所導(dǎo)致的心肌梗死大鼠的保護(hù)作用。方法 結(jié)扎左冠狀動脈前降支60 min 后進(jìn)行再灌注，與正常Ⅱ?qū)?lián)心電圖（ECG-Ⅱ）比較，冠脈結(jié)扎后10 min，ECG-Ⅱ的ST 段抬高0.2 mV 以上者作為缺血成功；再灌注后10 min，ECG-Ⅱ中抬高的ST 段下降30%以上者作為再灌注成功。造模成功后隨機(jī)分為模型組，能量合劑組（3.6 mg·kg^-1·d^-1）；另設(shè)8 只大鼠作為假手術(shù)組。給藥30 d 后測定彩色多普勒超聲心動圖、血流動力學(xué)、心肌梗死范圍；ELISA 法檢測血清丙二醛（MDA）、超氧化物歧化酶（SOD）、肌酸激酶同工酶（CK-MB）、肌酸脫氫酶（LDH）及心肌肌鈣蛋白（cTnT）的表達(dá)。結(jié)果 能量合劑可以改善心肌梗死大鼠的左心室功能，明顯提高左心室的射血分?jǐn)?shù)、降低左室舒張末壓，減少心肌梗死范圍，表明能量合劑能清除氧自由基，減少脂質(zhì)過氧化產(chǎn)物的生成，從而起到保護(hù)損傷心肌的作用。結(jié)論 能量合劑對心肌缺血再灌注損傷所導(dǎo)致的心肌梗死具有一定的保護(hù)作用。

Objective To investigate the therapeutic effects of energy mixture agent on myocardial infarction induced by myocardial ischemia-reperfusion in rats. Methods The acute myocardial infarction rat model was induced by ligation of the left anterior descending coronary artery for 60 min followed by reperfusion. The rats in which ST segment of Ⅱ lead electrocardiogram (ECG) was 0.2 mV more than the normal were as myocardial ischemia; The increased ST segment of Ⅱ lead ECG was reduced by 30% were as myocardial schemia-reperfusion. The model rats were divided into three groups randomly: model, energy mixture agent 3.6 mg·kg^-1·d^-1, and Sham group. The color Doppler echocardiography, cardiac hemodynamics, and range of myocardial infarction were measured; The blood levels of MDA, SOD, LDH, CK-MB, and cTNT were detected by ELISA after dosing for 30 d. Results Energy mixture agent could improve the left ventricular function in myocardial infarction rats, improve significantly the left ventricular ejection fraction, and reduce the left ventricular end-diastolic pressure and range of myocardial infarction, which suggested that energy mixture agent can scavenge the oxygen-free radicals and reduce the lipid peroxidative product-damaged heart tissue. Conclusion Energy mixture agant has significant protective effects on myocardial infarction induced by myocardial ischemia-reperfusion in rats.