目的 研究下瘀血湯中大黃生熟互換對熱結(jié)血瘀模型大鼠血管內(nèi)皮功能及微循環(huán)的影響。方法 采用ig 熱性中藥結(jié)合sc 鹽酸腎上腺素的方法，復(fù)制熱結(jié)血瘀大鼠模型。大鼠ig 熱性中藥28 d，第22 天起sc 鹽酸腎上腺素，并ig 相應(yīng)的藥物。第29 天，眼眶取血檢測檢測血管內(nèi)皮素（ET）、一氧化氮（NO）、前列環(huán)素（PGI₂）、血管性血友病因子（vWF）；股動脈取血，檢測血液流變學(xué)各項指標(biāo)。結(jié)果 含生大黃（復(fù)方A）和熟大黃（復(fù)方B）下瘀血湯均具有改善熱結(jié)血瘀模型大鼠血液流變學(xué)、血管內(nèi)皮細胞損傷和微循環(huán)的作用；與復(fù)方A 相比，復(fù)方B 各劑量組對血液流變學(xué)各指標(biāo)、ET、NO、PGI₂、vWF 因子水平具有顯著的改善作用（P<0.05、0.01）。結(jié)論 含熟大黃下瘀血湯的活血化瘀作用更強，可能是通過調(diào)節(jié)ET、NO、PGI₂、vWF 因子水平而實現(xiàn)的。

Objective To study the effect of raw/stewed Rhubarb in Xiayuxue Decoction on vascular endothelial function and microcirculation of rats with heat accumulation of blood stasis Methods Seventy SD rats were divided into seven groups. The rats in experimental groups were given heat drugs of traditional Chinese medicine for 28 d, then injected adrenaline hydrochloride for later 7 d, twice a day. At the same time, those experimental rats were given different doses of Xiayuxue Decoction (9 and 4.5 g·kg^-1) orally for 7 d. Then blood rheologic parameters such as ET, NO, PGI₂, and VWF in rats were detected. Results Xiayuxue Decoction A/B have improved vascular endothelial cell injury and the microcirculation of the rats with heat accumulation of blood stasis. Compared with Xiayuxue Decoction A, each dose of Xiayuxue Decoction B has improved the levels of ET, NO, PGI₂, and vWF factor significantly (P < 0.05, 0.01). The efficiency on promoting blood circulation to remove blood stasis of Xiayuxue Decoction B was definitely better than Xiayuxue Decoction A. Conclusion The mechanism of promoting blood circulation to remove blood stasis may be through regulating the levels of ET, NO, PGI₂, and VWF factor.

中醫(yī)藥行業(yè)科研專項（20110700710）