目的 觀察杜仲提取物對(duì)缺血再灌注損傷大鼠腦組織中腫瘤壞死因子-α(TNF-α)、白細(xì)胞介素-1β(IL-1β)及誘導(dǎo)型一氧化氮合酶(iNOS)mRNA表達(dá)的影響。方法 采用大鼠大腦中動(dòng)脈線栓法制備局灶性腦缺血再灌注損傷模型,造模前以杜仲提取物不同劑量連續(xù)ig給藥14 d,缺血2 h,再灌注24 h后,觀察杜仲提取物對(duì)腦缺血再灌注損傷大鼠神經(jīng)功能癥狀,腦梗死范圍改變,實(shí)時(shí)熒光定量PCR(RT-PCR)法檢測腦缺血再灌注損傷大鼠海馬TNF-α、IL-1β及iNOS mRNA的表達(dá)。結(jié)果 杜仲提取物可顯著改善腦梗死范圍(P<0.05),下調(diào)海馬TNF-α、IL-1β及iNOS mRNA的表達(dá)(P<0.05)。結(jié)論 杜仲提取物可下調(diào)大鼠腦組織中TNF-α、IL-1β及iNOS mRNA的表達(dá)產(chǎn)生抗局灶性腦缺血再灌注損傷作用。

Objective To investigate the effects of Eucommiae Cortex extracts on tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and inducible nitric oxide synthase (iNOS) mRNA expression in cerebral ischemia-reperfusion injury of rats. Methods The cerebral ischemia-reperfusion injury was induced by thread block of the middle cerebral artery. Eucommiae Cortex extracts were ig administrated once daily for consecutive 14 d pretreatment. After 24 h of ischemia and reperfusion, the neurologica1 deficit score was assessed and the brain tissues were obtained for detecting. The morphology of the brain tissues was observed with 2, 3, 5-triphenyl tetrazolium chloride (TTC) staining. Real time PCR was used to determine the mRNA expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and inducible nitric oxide synthase (iNOS). Results Compared with the model group, Eucommiae Cortex extracts ccould significantly decrease the brain infarction range and down-regulate the expression of TNF-α, IL-1β and iNOS mRNA. Conclusion Eucommiae Cortex extracts have the protective effects on focal brain ischemia reperfusion injury in rats by down-regulatting the expression of TNF-α, IL-1β, and iNOS mRNA.

貴州省中醫(yī)藥管理局資助項(xiàng)目(黔中醫(yī)藥發(fā) [2011] 78號(hào))