目的 優(yōu)選和胃顆粒中檳榔堿的最佳提取工藝。方法 應用單因素試驗考察藥材飲片的浸泡時間及吸水率,以檳榔堿提取率和出膏率的綜合評分為指標,通過正交試驗考察加水量、煎煮時間、煎煮次數對和胃顆粒提取工藝的影響,并采用高效液相色譜法測定檳榔堿的含量及其溶出度。結果 和胃顆粒中檳榔堿的最佳提取工藝為加水煎煮2次,第一次加水6倍量,煎煮1 h,趁熱濾過;第二次加水5倍量,煎煮1 h,趁熱濾過,合并濾液。結論 該提取工藝合理、可靠,適用于和胃顆粒的工業(yè)生產。

Objective To optimize the extraction technology for arecoline from Hewei Granules. Methods The method of single factor test was used to inspect soaking time and water absorption, with the yield of extract and the content of arecoline as indexes. Orthogonal test was adopted to investigate the influence of the amount of water, decocting time and frequences. HPLC was adopted to determine the content and dissolution of arecoline. Results The best extraction technology for arecoline from Hewei Granules was as follows: to be decocted twice, six times the amount of water for the first time, decoction for 1 h, hot filtration; five times the amount of water for the second time, decoction for 1 h, hot filtration and the filtrate was combined. Conclusion The extraction technology is reasonable, reliable, and suitable for the industrial production of Hewei Granules.