目的 建立救心滴丸中華蟾酥毒基、脂蟾毒配基的定量方法。方法 采用高效液相色譜法,以C₁₈色譜柱(250 mm×4.6 mm,5 μm);0.5%磷酸二氫鉀溶液-乙腈(50:50)(用磷酸調(diào)pH值為3.2)為流動(dòng)相;體積流量為1.0 mL/min,檢測(cè)波長(zhǎng)為296 nm。結(jié)果 華蟾酥毒基在0.092~1.472 μg呈良好的線性關(guān)系,r=0.999 7。平均回收率為101.4%,RSD值為1.0%。脂蟾毒配基在0.102~1.632 μg呈良好的線性關(guān)系,r=0.999 8。平均回收率為98.4%,RSD值為0.31%。結(jié)論 該方法簡(jiǎn)單、靈敏度高,可用于救心滴丸中華蟾酥毒基、脂蟾毒配基的定量測(cè)定。

Objective To develop an HPLC method for the determination of cinobufagin and resibufogenin in Jiuxin Dropping Pills. Methods The C₁₈ colum (250 mm×4.6 mm, 5μm) was used with 0.5% potassium dihydrogen phosphate-acetonitrile (pH=3.2) (50:50) as a mobile phase. The flow rate was 1.0 mL/min. The detecting wavelength was 296 nm. Results Cinobufagin: the linear range of ferulic acid was 0.092—1.472 μg, r=0.9997, the average recovery rate was 101.4%. Resibufogenin: The linear range of ferulic acid was 0.102—1.632 μg, r=0.9998. The average recovery rate was 98.4%. Conclusion The method is simple, rapid, and accurate for the determination of cinobufagin and resibufogenin in Jiuxin Dropping Pills.