目的 建立HPLC法測(cè)定龍加通絡(luò)膠囊中薯蕷皂苷。方法 采用Diamonsil C₁₈(4.6 mm×250 mm,5 mm)色譜柱,流動(dòng)相為乙腈-水(55:45),體積流量1.0 mL/min,設(shè)置溫度90 ℃,氣流量2.4 L/min,柱溫35 ℃。結(jié)果 薯蕷皂苷在1.03~6.18 μg與峰面積呈良好的線性關(guān)系(r=0.999 8),平均回收率100.96%,RSD=1.97%。結(jié)論 本方法簡(jiǎn)便、準(zhǔn)確、重復(fù)性好,可用于龍加通絡(luò)膠囊中薯蕷皂苷的質(zhì)量控制。

Objective To establish a method for the content determination of dioscin in Longjia Tongluo Capsule by HPLC. Methods The Diamonsil-C₁₈ (250 mm ×4.6 mm, 5 mm) column was used. The mobile phases consisted of acetonitrile and water (55:45); The flow rate was 1.0 mL/min, the set temperature was 90 ℃, the gas flow was 2.4 L/min, and the column temperature was 35 ℃. Results The linear range of dioscin was 1.03—6.18 μg (r=0.999 8), the average recovery was 100.96%, with RSD of 1.97%. Conclusion The method is simple, accurate, and reproducible, and it can be used in the quality control in Longjia Tongluo Capsule.