目的 研究龍血竭及其總黃酮類成分對慢性血瘀證兔模型的血液流變學指標及心主動脈內(nèi)皮細胞等的影響。方法 建立慢性血瘀證兔模型, 造模同時各給藥組每天給藥1次, 干預4周后對各組血液流變學各項指標以及血清一氧化氮(NO)和內(nèi)皮素(ET)水平進行檢測, 并通過HE染色病理切片對兔心主動脈內(nèi)皮細胞形態(tài)學變化進行觀察比較。結(jié)果 與模型組相比, 龍血竭及其總黃酮類成分均能降低慢性血瘀證兔模型全血黏度、血漿黏度、紅細胞壓積、紅細胞聚集指數(shù)和卡森黏度, 能升高紅細胞變形指數(shù), 對血小板聚集有抑制作用;同時能降低慢性血瘀證兔模型血清ET水平, 升高NO水平;此外, 各給藥組兔心主動脈HE染色病理切片內(nèi)皮細胞較模型組結(jié)構(gòu)完整而光滑, 排列平行有序, 損失程度有所改善。結(jié)論 龍血竭及其總黃酮類成分均能改善慢性血瘀證兔模型的血液流變學特性, 調(diào)節(jié)NO/ET平衡, 并對其心主動脈內(nèi)皮細胞有保護作用。

Objective This study is designed to explore the effect of Sanguis draxonis and its total flavonoids on hemorheology index and the heart aortic endothelial cells in the rabbit model with chronic stasis syndrome. Methods The rabbit-models of chronic stasis syndrome were established. At the beginning of the model making, each dosage group was dosed once daily. Four weeks later, the indexes of hemorheology, rabbit serum nitric oxide (NO), and endothelin (ET) level were detected, and the rabbit heart aorta tissue of endothelial cells morphological changes were observed by HE staining. Results Compared with the model group, both Sanguis draxonis and its total flavonoids can reduce whole blood viscosity, plasma viscosity, erythrocyte deposited, casson viscosity, and erythrocyte aggregation index, increase erythrocyte deformation index, and inhibit the platelet aggregation in the rabbit model with chronic stasis syndrome; At the same time, it also can reduce the serum levels of ET while increase the NO level in the rabbit model with chronic stasis syndrome. In addition, each dose group of endothelial cell structures in rabbit heart aorta of HE staining were complete, smooth, parallel arranged orderly, and loss rates were improved, compared with model group. Conclusion Both Sanguis draxonis and its total flavonoids can obviously improve blood rheological properties,regulate the balance of NO/ET, and protect the aortic endothelial cells in the rabbit model with chronic stasis syndrome.

"十二五"國家科技支撐計劃(2012BAI27B06)