目的 對(duì)不同干燥方式所得地龍氨基酸組分提取物進(jìn)行指紋圖譜測(cè)定方法的研究, 比較不同干燥方式對(duì)地龍氨基酸組分提取物品質(zhì)的影響。方法 采用異硫氰酸苯酯柱前衍生化反相高效液相色譜法, 測(cè)定并比較真空冷凍干燥、真空減壓干燥和微波干燥3種方式所得地龍干燥樣品以及未經(jīng)干燥樣品的指紋圖譜, 以中藥色譜指紋圖譜相似度評(píng)價(jià)系統(tǒng)計(jì)算相似度, 并進(jìn)行圖譜比較和指認(rèn)分析。結(jié)果 建立了不同干燥技術(shù)處理的地龍氨基酸組分提取物高效液相指紋圖譜, 確定了17個(gè)共有峰, 同一干燥方法不同批次干燥產(chǎn)品的鏡像度較高, 具有良好的相似性(>0.9), 不同干燥方法所得提取物之間有較大的質(zhì)量差異。結(jié)論 HPLC指紋圖譜分析法可較全面的反映不同干燥技術(shù)處理的地龍氨基酸組分提取物間的差異, 可用于同類提取物干燥工藝的質(zhì)量控制和工藝評(píng)價(jià)。冷凍干燥法是地龍氨基酸組分提取物最適宜的干燥方法。

Objective To study HPLC fingerprint methods of Pheretima amino acid components extract obtained by different drying methods and compare the effect of different drying methods on the quality of Pheretima amino acid components extract. Methods After derivatization of amino acids in Pheretima extract with taking phenyl isothiocyanate as derivatization reagent, the amino acids were analyzed by HPLC fingerprint technology. Pheretima extract was dried by vacuum freeze-drying, vacuum drying, and microwave drying. The similarity of chromatograms was calculated using a professional software named Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Chinese Pharmacopoeia Committee Version 2004A). Results Seventeen common peaks were identified in the Pheretima amino acid components extract by HPLC fingerprint. The chromatograms of different batches of product dried by the same drying methods present a good similarity (> 0.9). There are certain quality differences in the extract by the different drying methods. Conclusion The quality control and process evaluation of herbal extracts by HPLC fingerprint technology are simple and reliable. The optimal drying method for Pheretima extract is vacuum freeze-drying.