在藥物分析中高效液相色譜法用得非常普遍, 而色譜柱的選擇直接影響實(shí)驗(yàn)結(jié)果與藥物的質(zhì)量控制。不同C18色譜柱的保留行為千差萬(wàn)別, 選擇適宜的色譜柱對(duì)分析樣品至關(guān)重要。以C18色譜柱的內(nèi)部化學(xué)鍵合相為切入點(diǎn), 介紹C18色譜柱的差異, 并綜述在不同的pH環(huán)境、流動(dòng)相極性、藥物極性等條件下以及根據(jù)色譜柱的分類系統(tǒng)選擇合適的鍵合相, 為如何正確快速的選擇C18色譜柱提供參考。

HPLC is very popular in the analysis of drugs. while the column selection affects the experimental results. The retention behavior of different C18 chromatographic column differ in thousands of ways, choose the appropriate column is essential to analysis of samples. From the internal chemical bonds combination with C18 chromatographic column, introduce different C18 column. Overview how to select bonded phase in different pH environment, different mobile phase polarity,different drug polarity conditions. And according to the classification system of chromatographic column select bonded phase. Provides the reference for the C18 column selection.