目的 研究大鼠腦缺血再灌注后,血栓通主要成分人參皂苷Rb₁、Rg₁、Re、Rd及三七皂苷R₁在腦內(nèi)的分布狀況。方法 采用超高效液相色譜質(zhì)譜聯(lián)用法(UPLC-MS/MS)測(cè)定給予不同濃度的血栓通后,不同時(shí)間點(diǎn)血栓通主要成分在正常腦組織和缺血腦組織中的含量。結(jié)果 結(jié)果表明,血栓通活性成分在腦缺血大鼠腦中的分布遠(yuǎn)高于正常大鼠,并且其在腦內(nèi)的含量隨再灌注時(shí)間的延長(zhǎng)降低。結(jié)論 腦缺血再灌注后,血腦屏障開放,注射用血栓通(凍干)可以透過血腦屏障發(fā)揮作用。

Objective To study the intracephalic distribution of ginsenosides Rb₁, Rg₁, Re, Rd and notoginsenoside R₁ from Xueshuantong Injection (lyophilized powder) after ischemia-reperfusion. Methods Using ultra performance liquid chromatography tandem massspectrometry (UPLC-MS/MS), the contents of active ingredients in Xueshuantong Injection in normal brain tissue and ischemic tissue at different drug concentration and time points were measured. Results The distribution of active ingredients of Xueshuantong Injection in cerebral ischemia brain was much higher than that of normal ones, and the content of active ingredients in brain reduces with reperfusion prolonged. Conclusion Xueshuantong Injection plays its role because blood brain barriar opens after ischemia-reperfusion.

國(guó)家重大新藥創(chuàng)制(2012ZX09101201-004);高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金(20101210110002);天津市應(yīng)用基礎(chǔ)與前沿技術(shù)研究計(jì)劃(14JCYBJC28900);廣西科學(xué)研究與技術(shù)開發(fā)計(jì)劃(14125008-2-5)