目的 探討潤燥清肺膏的止咳、抗炎、抑菌的藥效學(xué)作用。方法 將50 只小鼠及豚鼠各隨機(jī)分為5 組,分別為模型組、陽性對照藥急支糖漿組和潤燥清肺膏高、中、低劑量組,采用氨水噴霧致咳小鼠模型及枸櫞酸噴霧致咳豚鼠模型評價止咳作用;將60 只大鼠隨機(jī)分為6 組,分別為正常組、模型組、陽性對照藥急支糖漿組和潤燥清肺膏高、中、低劑量組,采用脂多糖致急性支氣管炎模型大鼠評價抗炎作用;將50 只小鼠隨機(jī)分為5 組,分別為模型組、陽性對照藥急支糖漿組和潤燥清肺膏高、中、低劑量組,采用醋酸致小鼠腹腔毛細(xì)血管通透性增高及巴豆油致小鼠耳腫脹等模型評價其抗炎作用;采用體外抑菌實驗,評價其抑菌作用。結(jié)果 潤燥清肺膏高、中、低各劑量組豚鼠咳嗽潛伏期明顯延長(P< 0.05、0.01)、咳嗽次數(shù)明顯減少(P< 0.01);潤燥清肺膏能減輕急性支氣管炎大鼠模型黏膜上皮細(xì)胞的壞死和脫落,抑制黏膜層和黏膜下層炎性細(xì)胞浸潤;潤燥清肺膏高、中劑量組明顯抑制醋酸所致小鼠腹腔毛細(xì)血管通透性的增高(P< 0.05、0.01);潤燥清肺膏高、中、低各劑量組明顯地抑制小鼠耳腫脹(P< 0.05、0.01)。潤燥清肺膏對金黃色葡萄球菌、表皮葡萄球菌、肺炎克雷伯菌和大腸桿菌有不同程度的抑制作用。結(jié)論 潤燥清肺膏對急性支氣管炎模型大鼠支氣管病變有抑制作用,具有明顯的止咳、抗炎、抑菌作用。

Objective To observe the main effects of the Ruzaoqingfei Gao in the treatment on asthma, cough and anti-inflammator. Methods Fifty rats were divided into 5 groups, the normal group, Ruzaoqingfei Gao high, middle, low, jizhi yrup, by with ammonia water spray determination of cough incubation period and Citrate spray induced cough model of guinea pigs; 60 big mice divided into 6 group, the normal group Ruzaoqingfei Gao high, middle, low dose group, jizhi syrup group or ammonium chloride, with the rat model of chronic bronchitis was induced with intratracheal instillation of lipopolysaccharide to observe the lung pathematology; 50 mice were divided into 5 groups, the normal group, Ruzaoqingfei Gao high, middle, low dose group, jizhi syrup group, acute inflammation model caused by dimathyl benzene and albumen.To evaluat bacteriostasis in vitro bacteriostasis experiment. Results Ruzaoqingfei Gao with high, medium and low dose groups of guinea pig cough obviously prolong the incubation period (P < 0.05, P < 0.01), less cough obviously (P < 0.01); The histopathological examination revealed Ruzaoqingfei Gao could reduce the chronic bronchitis rats with varying degrees of bronchial mucosa and submucosa infiltration of inflammatory cells, and reduce airway secretions; Ruzaoqingfei Gao with high, middle dose group significantly inhibited the increase of blood capillary permeability of the abdominal in mice caused by acetic acid (P < 0.05, P < 0.01); Ruzaoqingfei Gao with high, medium and low dose group obviously inhibit ear swelling in mice (P < 0.05, P < 0.01). With Ruzaoqingfei Gao on staphylococcus aureus and epidermis staphylococcus aureus, klebsiella pneumonia and escherichia coli, shows that there are different degrees of inhibition. Conclusion we confirmed the effects such as antitussive,anti-asthma and anti-inflammatory on animals of Ruzaoqingfei Gao.

山東省科技發(fā)展計劃項目(2014GSF119039)