目的 建立測定不同產地西洋參藥材及其產品中10 種皂苷成分含量的方法,比較不同來源西洋參中總皂苷及單體皂苷的含量差異,評價品質優(yōu)劣。方法 采用高效液相色譜法,色譜條件為Thermo Scientific Syncronis C₁₈ 色譜柱(100 mm×2.1mm,1.7 μm),流動相為乙腈-水,二元梯度洗脫,體積流量為0.2 mL/min,檢測波長為203 nm,柱溫為35 ℃。結果 人參皂苷Rg₁、Re、Rb₁、Rc、Rb₂、Rd、F₁、R₀、F₂、Rk₁ 分別在線性范圍內存在良好的線性關系,48 h 內穩(wěn)定性試驗RSD 值均小于5%,平均加樣回收率為96%～102%,RSD 值均< 2.21%。結論 該方法準確、快速、靈敏,重復性好,可用于西洋參藥材、飲片及相關產品中皂苷的定量分析。不同來源西洋參及其產品中皂苷成分的種類相似,但單體皂苷的含量差異顯著,所測樣品均不含有人參皂苷Rf,排除摻假。

Objective To establish the method to determine the contents of 10 ginsenoside compounds in Panax quinquefolium from different origins. And the contents of total ginsenosides and monomer ginsenosides in samples from different origins were compared. The quality of different sample was evaluated. Methods Thermo Scientific Syncronis C18 (2.1 mm × 100 mm, 1.7 μm) was used with a gradient of acetonitrile and 0.1% formic acid at a flow rate of 0.2 mL/min. The detection wavelength was set at 203 nm and the column temperature was at 35 ℃ . Results Ginsenoside Rg₁, Re, Rb₁, Rc, R₀, Rb₂, Rd, F₁, F₂, and Rk₁ were in a good linear relationship within the linear range, respectively. And the average RSD value of stability test is less than 5% within 48 h. The average sample recovery was between 96%—102%. Conclusion This method is accurate, rapid, and sensitive and with good repeatability. The method can be used for quantitative analysis of ginsenosides in P. quinquefolium materials, decoction pieces, and related products. The types of ginsenoside constituents in samples from different origins were similar and the contents of monomer ginsenosides were significantly different. No ginsenoside Rf was found in the detected samples and eliminated the adulteration.

公益性行業(yè)(農業(yè))科研專項經費項目(20130311106)