目的 研究注射用苦參堿對(duì)阿霉素引起大鼠心臟重構(gòu)的影響。方法 將52只大鼠隨機(jī)分為:對(duì)照組、模型組、苦參堿(100 mg/kg)組,尾iv阿霉素制備心肌損傷模型,采用超聲心動(dòng)圖檢測(cè)大鼠心臟左心室舒張末期內(nèi)徑(LVEDd)、左室射血分?jǐn)?shù)(LVEF)和縮短分?jǐn)?shù)(FS);生理記錄儀檢測(cè)大鼠左心室舒張末期壓(LVEPD)、收縮壓(LVPS)、室內(nèi)壓最大收縮/舒張速率(±dp/dt_max);稱大鼠體質(zhì)量,脊髓離斷法處死大鼠,稱大鼠心臟質(zhì)量并計(jì)算心臟指數(shù);試劑盒法檢測(cè)大鼠心臟組織中谷胱甘肽過(guò)氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)水平;應(yīng)用流式細(xì)胞術(shù)檢測(cè)大鼠心肌細(xì)胞凋亡水平。結(jié)果 與模型組比較,苦參堿組大鼠LVEDd值顯著降低、EF和FS值顯著升高;LVEPD顯著降低,LVPS、dp/dt_max及－dp/dt_max均顯著升高;心臟指數(shù)、心肌細(xì)胞凋亡率顯著降低;心肌細(xì)胞內(nèi)GSH-Px、SOD活性明顯升高,MDA水平顯著降低。結(jié)論 注射用苦參堿減輕阿霉素引起的心肌細(xì)胞凋亡,改善心臟重構(gòu)及心臟功能,可能與改善心肌細(xì)胞氧化應(yīng)激水平有關(guān)。

Objective To study the regulation of matrine on adriamycin-induced cardiac remodeling in rats. Methods Sixty SD rats were randomly divided into control group, model group, and matrine group. The cardiac LVEDd, LVEF, and FS values of three groups were detected by echocardiography. The values of LVEPD, LVPS, ±dp/dt_max were detected by physiological recorder. The weights of rats were measured. The rats were killed by the method of spinal cord transection, the weight of the heart of rat was measured and the heart index was calculated. The levels of oxidative stress were evaluated by using reagent kit of glutathione peroxidase (GSH-Px), total superoxide dismutase (SOD), and malondialdehyde (MDA). Flow cytometry is used to test the apoptosis of rat myocardial cells. Results Compared with model group, the value of LVEDd in matrine group decreased significantly, the value of EF and FS increased significantly; The value of LVEPD decreased significantly, the values of LVPS, dp/dt_max, and -dp/dt_max increased significantly; The heart index and myocardial apotosis levels significantly decreased; The expression levels of SOD and GSH were decreased, and MDA content was significantly increased. Conclusion Matrine reduced the apoptosis of cardiac cells induced by adriamycin in rats and improved cardiac remodeling and function which changes may be caused by oxidative stress levels improvement.

黑龍江省衛(wèi)生廳科研項(xiàng)目(2014-267);佳木斯大學(xué)科研項(xiàng)目(S2014-004)