目的 研究刺老苞Araliae Echinocaulis Radicis et Cortex根皮總提取物對去勢雌性大鼠骨質(zhì)疏松癥的影響。方法 75只SD雌性大鼠隨機分成對照組、假手術(shù)組、模型組、仙靈骨葆膠囊組和刺老苞根皮總提取物組,每組15只。經(jīng)去勢手術(shù)2周后,仙靈骨葆膠囊組、刺老苞根皮總提取物組ig相應(yīng)藥物懸液(20 mg/kg),每天1次,連續(xù)12周。觀察大鼠給藥前后體質(zhì)量變化;試劑盒法檢測血清總膽固醇(TC)、高密度脂蛋白-膽固醇(HDL-C)、低密度脂蛋白-膽固醇(LDL-C)、堿性磷酸酶(ALP)水平;取右側(cè)股骨檢測骨密度、骨微量元素和羥脯氨酸(Hyp)水平;取左側(cè)股骨進行骨力學(xué)測定。結(jié)果 與對照組比較,模型組大鼠血清TC、LDL-C水平均顯著升高(P<0.01),血清HDL-C、ALP、骨微量元素(鈣、磷、鎂、錳)、Hyp水平,骨密度、骨力學(xué)指標(biāo)均顯著下降(P<0.01);與模型組比較,刺老苞根皮總提取物組與仙靈骨葆膠囊組TC、LDL-C水平均顯著降低(P<0.05),血清HDL-C、ALP、骨微量元素(鈣、磷、鎂、錳)、Hyp水平,骨密度、骨力學(xué)指標(biāo)均顯著升高(P<0.05)。結(jié)論 刺老苞根皮總提取物有效改善去勢雌性大鼠骨質(zhì)疏松癥的發(fā)生。

Objective To study the effect of total extract from Araliae Echinocaulis Radicis et Cortex on osteoporosis in ovariectomized rats. Method SD rats (75 cases) were randomly divided into five groups, including control group, Sham-operation group, model group, Xiangling Gubao group and Araliae Echinocaulis Radicis et Cortex total extract group. Two weeks after ovarectomized operation, the rats in Xiangling Gubao group and Araliae Echinocaulis Radicis et Cortex total extract group were fed respectively by 20 mg/kg dosage once daily for 12 weeks. Rats weight changes were observed; The levels of total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), and alkaline phosphatase (ALP) were investigated by related reagent kit; The bone mineral density (BMD), bone trace elements, and hydroxyproline (Hyp) of right femur, and bone mechanics of left femur were measured. Result Compared with control group, serum TC and LDL-C levels were increased (P < 0.01), but serum HDL-C and ALP levels, bone microelement (Ca/P/Mg/Mn) and Hyp contents, BMD and bone mechanics indexes were decreased in model group (P < 0.01). However, compared with model group, serum TC and LDL-C levels were decreased (P < 0.05), but serum HDL-C and ALP levels, bone microelement (Ca/P/Mg/Mn) and Hyp contents, BMD and bone mechanics indexes were increased in Xiangling Gubao group and Araliae Echinocaulis Radicis et Cortex total extract group (P < 0.05). Conclusion Araliae Echinocaulis Radicis et Cortex total extract may inhibit effectively osteoporosis of ovariectomized rats.

國家自然科學(xué)基金資助項目(81173361、81270051、81473451)