目的 研究并建立加味逍遙提取物(柴胡、梔子、丹皮、白芍、當(dāng)歸、白術(shù)、茯苓、甘草、薄荷和干姜)的超高效液相色譜指紋圖譜,為加味逍遙提取物質(zhì)量控制提供簡(jiǎn)便、可靠方法。方法 采用超高效液相色譜法,以UPLCTM HSS T3色譜柱(100 mm×2.1 mm,1.8 μm)為分析柱,乙腈-0.05%磷酸水溶液為流動(dòng)相,梯度洗脫,柱溫30℃,體積流量為0.3 mL/min,檢測(cè)波長(zhǎng)238 nm。結(jié)果 加味逍遙提取物中多數(shù)峰可以達(dá)到基線分離。用中藥色譜指紋圖譜相似度評(píng)價(jià)系統(tǒng)2012年版對(duì)10批樣品的指紋圖譜進(jìn)行峰匹配,確定34個(gè)共有峰,10批加味逍遙提取物中共有峰的相對(duì)保留時(shí)間RSD值小于1%,指紋圖譜的相似度均在0.97以上,選取共有峰在單味藥材中除茯苓、柴胡、白術(shù)外,均找到歸屬。結(jié)論 該法建立的指紋圖譜穩(wěn)定性好,靈敏度高,可作為加味逍遙提取物的質(zhì)量控制方法,并為其復(fù)雜成分的研究提供參考和依據(jù)。

Objective To provide a sensitive method for evaluation of extract of Modified Xiaoyao (Bupleuri Radix, Cape jasmine, Paeonol, Paeoniae, Alba Radix, Angelicae Sinesis Radix, Atractylodis Macrocephalae Rhizoma, Poria, Glycyrrhizae Radix et Rhizoma, Menthae haplocalycis Herba, and Zingiberis Rhizoma Praeparatum) by UPLC fingerprint. Methods UPLC was performed on UPLCTM HSS T3 column (100 mm×2.1 mm, 1.8 μm). The mobile phase was a gradient of acetonitrile-0.05 % phosphoric acid. The detection wavelength was set at 238 nm. The flow rate was 0.3 mL/min and column temperature was 30 ℃. Results Most peaks could be separated preferably, and the RSD values of the relative relation time of 10 batches of extract of Modified Xiaoyao were lower than 1%, 34 common peaks were obtained in the fingerprint of 10 batches of extract of Modified Xiaoyao with the help of peaks match with Similarity Evaluation System for Chromatographic Fingerprint of TCM. The similarity among fingerprints was calculated more than 0.97. The common peaks selected belonged to all the ingredients that could be used to classify the species, but Poria, Bupleuri Radix and Atractylodis Macrocephalae Rhizoma did not share common peaks. Conclusion The fingerprint could be used as a method of quality control and provides evidence for the research of effective components of Xiaoyao Powder.