目的 研究黑木耳多糖（AAP）和松多酚（PKP）聯(lián)合使用對⁶⁰Coγ輻射誘導(dǎo)小鼠氧化損傷的修復(fù)作用。方法 昆明小鼠除對照組外⁶⁰Coγ射線一次性全身均勻照射，總吸收劑量為4.0 Gy，吸收劑量率為1.0 Gy/min。輻射24 h后分別設(shè)AAP組、PKP組、AAP與PKP協(xié)同（AP）組及茶多酚（TP）組，分別ig 75、25、37.5+12.5、25 mg/kg（以多糖或多酚水平計(jì)）相應(yīng)藥物，對照組和模型組ig等體積生理鹽水。連續(xù)喂養(yǎng)30 d后空腹24 h處死，測定小鼠的血清、脾臟、肝臟、心臟中維生素C（VC）、谷胱甘肽（GSH）、輔酶Q10（CoQ10）、丙二醛（MDA）水平和超氧化物歧化酶（SOD）、谷胱甘肽過氧化物酶（GSH-Px）活性；取小鼠無菌脾細(xì)胞進(jìn)行彗星電泳，熒光顯微鏡成像系統(tǒng)采集圖像。結(jié)果 輻射后給藥PKP、AAP、AP和TP均可一定程度上升高VC、GSH、CoQ10水平，升高SOD、GSH-Px活性，降低MDA水平，減小脾細(xì)胞彗星尾長度，其中AP組輻射后修復(fù)效果較好。結(jié)論 與AAP、PKP、TP組比較，AP不僅可以促進(jìn)體質(zhì)量的恢復(fù)，還能有效地清除體內(nèi)自由基，降低自由基對體內(nèi)DNA造成的損傷，顯著提高⁶⁰Coγ輻射誘導(dǎo)氧化損傷的修復(fù)能力。

Objective To study the effect of polysaccharides from Auricularia auricula (AAP) and polyphenols from Pinus koraiensis (PKP) combinations (AP) on recoveries of antioxidant injury induced by ⁶⁰Coγ radiation. Methods The model group and five experimental groups were irradiated once with ⁶⁰Coγ ray. AAP, PKP, AP, and TP had been given to mice by gavage for 30 d at doses of 75.0, 25.0, 37.5+12.5 and 25.0 mg/kg (calculate with polysaccharide or polyphenol levels), respectively. The mice in control and model groups were given normal saline by gavage during that time. After 30 d, contents of vitamin C (VC), glutathione (GSH), coenzyme Q10 (CoQ10), and malondialdehyde (MDA), and activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the serum, spleen, liver, and heart of mice were determined. Meanwhile, the nuclear DNA degradation in spleen was observed by comet assay. Results It showed that AAP, PKP, AP, and TP could significantly increase the contents of VC, GSH, CoQ10, improve the activities of SOD and GSH-Px, reduce MDA content, and decrease the comet tail lengths as compared to model group. Above all, effect of AP on antioxidant recovery was the best. Conclusion Compared to AAP, PKP, and TP groups, AP not only can faster pick up flesh, but also more effectively remove free radicals and reduce damages to DNA, more significantly improve antioxidant recovery from radiation induced oxidative injury.

國家自然科學(xué)基金資助項(xiàng)目（31170510）；中央高?；究蒲袠I(yè)務(wù)費(fèi)專項(xiàng)（DL11BA18）