目的 研究藏藥川西獐牙菜提取物對(duì)人胃癌細(xì)胞MGC-803增殖抑制作用和細(xì)胞周期的影響。方法 MTT法測定川西獐牙菜石油醚部位、醋酸乙酯部位、正丁醇部位、水部位和甲醇總提取物部位對(duì)人胃癌細(xì)胞MGC-803的增殖抑制作用；流式細(xì)胞術(shù)檢測正丁醇部位對(duì)細(xì)胞周期的影響；高效液相色譜-串聯(lián)質(zhì)譜（HPLC-MS/MS）法檢測正丁醇部位的主要化學(xué)成分。結(jié)果 藏藥川西獐牙菜各提取部位對(duì)人胃癌細(xì)胞MGC-803增殖均有抑制作用，正丁醇部位的增殖抑制效果最佳且具有明顯的量效關(guān)系；用正丁醇部位處理MGC-803細(xì)胞，表現(xiàn)出明顯的S期周期阻滯；HPLC-MS/MS檢測結(jié)果表明正丁醇部位的主要化學(xué)成分為1，7，8-三羥基-3-甲氧基呫噸酮、1，8-二羥基-3，5-二甲氧基呫噸酮、1，3，6，7-四羥基呫噸酮-2-β-D-葡萄糖、2’-間羥基苯甲酰獐牙菜苷、獐牙菜苷-6’-O-葡萄糖和1-羥基-2，3，5-三甲氧基呫噸酮-1-O-[β-D-吡喃木糖（1-6）-β-D-吡喃葡萄糖]。結(jié)論 川西獐牙菜各部位對(duì)人胃癌細(xì)胞MGC-803均具有增殖抑制作用，正丁醇部位的抑制作用最強(qiáng)且能夠通過改變細(xì)胞周期分布抑制人胃癌細(xì)胞MGC-803的生長，其中主要成分為呫噸酮類化合物。

Objective To investigate proliferation and cell cycle of MGC-803 cancer cells treated with butanol extracts of Tibetan medicine Swertia mussotii (BESM). Methods Proliferation of human gastric cancer MGC-803 cells treated with BESM were assayed by MTT in vitro, and the change of cell cycles treated with BESM was detected by flow cytometry. The main compounds of BESM have been analyzed by HPLC-MS-MS. Results BESM could inhibit the proliferation of human gastric cancer MGC-803 cells; After treating with BESM, MGC-803 cell cycle was arrested remarkably at S phase. The main compounds from BESM were 1,7,8-trihydroxy-3-methoxyxanthone, 1,8-dihydrox-3,5-dimethoxyxanthone, 1,3,6,7-tetrahydroxyxanthone-2-β-D-glucopyranosyl, 2'-m-hydroxybenzoylsweroside, 6'-O-glucopyranosylsweroside, and 1-O-[β-D-xylopyranosyl-(1→6)-β-D-glucopyranosyl]-3,5- dimethoxyxanthone. Conclusion BESM could inhibit the growth of human gastric cancer MGC-803 cells, and can be used to regulate MGC-803 carcinoma cells proliferation by inhibiting cells in S phase, and the main component of it is xanthone.

國家自然科學(xué)基金（11372162與11432008）