目的 觀察芒果三芪肺纖方對博萊霉素誘導(dǎo)小鼠肺纖維化的改善作用。方法 昆明小鼠隨機分為對照組，模型組，地塞米松（陽性藥，1 mg/kg）組，芒果三芪肺纖方高、中、低劑量（以生藥計5.00、3.30、1.65 g/kg）組，通過鼻腔1次性滴入6 mg/kg鹽酸博萊霉素制備小鼠肺纖維化模型，對照組滴入等體積的生理鹽水。于造模后第14天開始ig給藥，每天給藥1次，連續(xù)給藥14 d后處死小鼠，試劑盒法測定肺組織勻漿中的羥脯氨酸（HYP）、丙二醛（MDA）、白介素-1β（IL-1β）水平和超氧化物岐化酶（SOD）活力；HE染色觀察肺組織病理切片，Masson染色觀察膠原纖維的分布情況。結(jié)果 與模型組比較，芒果三芪肺纖方高、中、低劑量組小鼠HYP和IL-1β水平均顯著降低（P<0.01），SOD活力顯著提高（P<0.05、0.01），高和中劑量組小鼠MDA水平顯著降低（P<0.05、0.01）；HE和Masson染色顯示，芒果三芪肺纖方組肺泡炎損傷和肺纖維化病變程度明顯減輕（P<0.01），高劑量組效果最好。結(jié)論 芒果三芪肺纖方通過提高SOD活力、降低MDA水平，發(fā)揮抗自由基損傷作用；降低HYP的水平，減少纖維蛋白的形成；降低IL-1β水平，發(fā)揮抗炎作用；顯著減輕肺泡炎癥損傷、抑制肺纖維化病變程度，對博萊霉素誘導(dǎo)的小鼠肺纖維化具有很好的改善作用。

Objective To investigate the effect of Mangguosanqi Feixian Prescription on bleomycin-induced pulmonary fibrosis in mice. Methods Kunming mice were randomly divided into six groups: control group, model group, dexamethasone (1 mg/kg, positive drug) group, Mangguosanqi Feixian Prescription low, medium and high dose (5.00, 3.30, and 1.65 mg/kg) groups. Mice were prepared with intranasal instillation infusion with 6 mg/kg bleomycin, control group treated with the same volume of physiological saline. Mice in each treatment group were given appropriate intervention 14 d after modeling, and were sacrificed after 14 d. Reagent kit method was used to determine the levels of homogenate (HYP), malondialdehyde (MDA), and interleukin-1β (IL-1β), and the activity of superoxide enzyme (SOD) in lung tissue homogenate. Pathological sections were stained with haematoxylin-eosin and Masson dyeing for pathological diagnosis. Results Compared with model group, the level of HYP, IL-1β in Mangguosanqi Feixian Prescription low, medium, and high dose groups was decreased significantly, the activity of SOD was increased significantly, and the levels of MDA in medium and high dose groups were decreased significantly (P<0.05, 0.01). Mangguosanqi Feixian Prescription group could significantly relieve inflammation and restrain the degree of pulmonary fibrosis (P<0.01).High-dose group is the best. Conclusion Mangguosanqi Feixian Prescription can increase SOD activity, decrease MDA levels play against free radical injury, reduce the content of HYP, reduce the formation of fibrin, and regulate the expression of IL-1β to exert anti-inflammatory effects. It can also significantly reduce damage alveolitis and inhibition of pulmonary fibrosis disease. It has a very good therapeutic effect on bleomycin-induced pulmonary fibrosis in mice.

廣西“八桂學(xué)者”工程專項經(jīng)費資助（廳發(fā)[2013]3號）