目的 比較半夏白術(shù)天麻湯不同提取方法對(duì)大鼠血清中單胺類遞質(zhì)水平的影響。方法 原發(fā)性痰濕壅盛型高血壓大鼠，隨機(jī)分為模型組、半仿生-酶法提取液（SBEE）組、水提液（SBE）組、醇提液（WE）組、半仿生提取液（AE）組，4種提取液均過(guò)中空纖維膜截留相對(duì)分子質(zhì)量≤1 000。模型組飲用去離子水，不限量；SBEE組、SBE組、WE組、AE組分別ig相應(yīng)提取液，每天1 mL（2.83 g/mL）；正常血壓大鼠8只作為對(duì)照組。喂養(yǎng)4周后，采用高效液相色譜-電化學(xué)（HPLC-ECD）法測(cè)定血清中單胺遞質(zhì)去甲腎上腺素（NE）、5-羥色胺（5-HT）水平。結(jié)果 4種提取液均可降低高血壓大鼠NE和5-HT水平，與模型組比較差異顯著（P<0.01），對(duì)NE影響順序是：SBEE > SBE > AE > WE，對(duì)5-HT影響順序是：SBEE > SBE > WE > AE。結(jié)論 半夏白術(shù)天麻湯4種方法提取均可降低高血壓大鼠單胺遞質(zhì)水平，且加入酶解過(guò)程的SBEE提取方法效果最好。

Objective To investigate the effect of four extracting solutions in Banxia Baizhu Tianma Decoction (BBTD) with relative molecular mass≤1 000 on levels of monoamine neurotransmitters in rat plasma. Method Primary phlegm dampness type hypertensive rats were randomly divided into model group, SBEE group, SBE group, WE group, and AE group, respectively, by ig administration of corresponding semi-bionic enzymetic extract, water extract, alcohol extract, and semi-bionic extraction liquid, 1 mL (2.83 g/mL) daily. Rats with normal blood pressure were used as control group. After four weeks, the norepinephrine (NE) and 5-serotonin (5-HT) content of serum were determined by HPLC method. Results Compared with model group, totally four extracting solutions of BBTD could reduce NE and 5-HT levels significantly in hypertension rats (P<0.01), order of the effect was SBEE > SBE > AE > WE and SBEE > SBE > WE > AE, respectively. Conclusion Totally four extracting solutions of BBD can reduce monoamine transmitters in hypertension rats and SBEE extraction is better.

浙江省自然科學(xué)基金（No. LY16H270018）；國(guó)家科技部國(guó)際合作項(xiàng)目（2007DFA31180）