目的 研究黑木耳多糖（AAP）和紅松多酚（PKP）協(xié)同對⁶⁰Coγ輻射致小鼠免疫損傷的修復作用。方法 昆明小鼠除對照組外⁶⁰Coγ射線一次性全身均勻照射，總吸收劑量為4.0 Gy，吸收劑量率為1.0 Gy/min。輻射24 h后分別設(shè)AAP組，PKP組，AP（AAP與PKP協(xié)同）組及靈芝多糖（GLP）組，分別ig 75.0、25.0、37.5+12.5、75.0 mg/kg（以多糖或多酚水平計）相應(yīng)藥物，同時對照組和模型組小鼠ig等體積生理鹽水。連續(xù)喂養(yǎng)30 d后空腹24 h處死，測定小鼠的胸腺、肝臟、脾臟、心臟、腎臟和腦的器官指數(shù)；瑞士－吉姆薩染液染色測定正常外周血白細胞數(shù)量；碳廓清實驗檢測吞噬指數(shù)（α）；MTT法檢測脾淋巴細胞刺激指數(shù)（SI）。結(jié)果 輻射后給藥PKP、AAP、AP、TP和GLP都會一定程度升高小鼠肝臟、脾臟、心臟和胸腺的器官指數(shù)，增加正常外周血白細胞數(shù)量，升高α和SI，其中AP組對輻射后修復效果較好。結(jié)論 比較AAP、PKP、TP及GLP，AP顯著的結(jié)合了AAP和PKP的優(yōu)點，從特異性免疫和非特異性免疫對輻射后小鼠進行了最大限度的修復。

Objective To study the effect of polysaccharides from Auricularia auricula (AAP) and polyphenols from Pinus Koraiensis (PKP) combination (AP) on recoveries of immunologic injury induced by ⁶⁰Coγ radiation in mice. Methods The model group and five experimental groups were irradiated once with ⁶⁰Coγ ray. AAP, PKP, AP, and Ganoderma lucidum polysaccharides (GLP) had been given to mice by gavage for 30 d at doses of 75.0, 25.0, 37.5+12.5, and 75.0 mg/kg (calculated with polysaccharide or polyphenol levels), respectively. The control and model groups were given normal saline by gavage during that time. After 30 d, the organ indexes of thymus, liver, spleen, heart, kidney, and brain, normal white blood cell counts, phagocytosis indexes (α), and stimulation indexes (SI) of mice were determined. Results AAP, PKP, AP, and GLP could significantly increase the organ indexes of thymus, liver, spleen, heart; normal white blood cell counts, α, and SI. Above all, effect of AP on immune recovery was the best. Conclusion Compared to AAP, PKP, and GLP, AP could combine the advantages of AAP and PKP, preformed the best effect of specific immunity function and non-specific immunity function.

國家自然科學基金（31170510）資助項目；中央高校基本科研業(yè)務(wù)費專項（DL11BA18）