目的 觀察注射用血塞通（凍干）對電刺激所致家兔下腔靜脈血栓形成的影響，并對其作用機(jī)制進(jìn)行探討。方法 使用電刺激伴狹窄法制備家兔下腔靜脈血栓模型，造模1 h后，連續(xù)5天iv給予5、10、20 mg/kg的注射用血塞通（凍干）、sc給予100 U/kg的低分子肝素鈉注射液，最后一次給藥1 h后測定家兔出血時間和出血量，頸動脈取血分離血漿，試劑盒法檢測活化部分凝血活酶時間（APTT）、凝血酶原時間（PT）、凝血酶時間（TT）以及纖維蛋白原（FIa）水平，ELISA試劑盒法檢測血漿內(nèi)凝血因子FIIa、FXa、FXIa、抗凝血酶III（ATIII）、凝血因子TAT、活化蛋白C（APC）、組織性纖維溶酶激活劑（t-PA）、纖溶酶原（PLG）、尿激酶原（PROUK）、胰蛋白酶（Trypsin）、6-酮前列腺素F1a（6-keto-PGF1a）和血栓素B₂（TXB₂）水平；取血栓，測定血栓濕質(zhì)量、干質(zhì)量以及基質(zhì)量。在血小板聚集實驗中，制備家兔貧、富血小板血漿，分別以膠原（CG）、二磷酸腺苷二鈉鹽（ADP）和花生四烯酸鈉（AA）為誘導(dǎo)劑，比濁法測定不同濃度血塞通對血小板聚集的影響。結(jié)果 家兔iv注射用血塞通（凍干）后，與模型組比較，血栓濕質(zhì)量、干質(zhì)量及基質(zhì)量均顯著降低，并具有一定的劑量依賴性；出血時間和出血量未出現(xiàn)明顯增加，APTT、PT和TT也未出現(xiàn)明顯變化；血漿內(nèi)t-PA和6-keto-PGF1a水平顯著增加，TXB₂水平顯著降低，其他指標(biāo)沒有明顯變化。在血小板聚集實驗中，血塞通可以劑量依賴性地抑制由AA誘導(dǎo)的血小板聚集。結(jié)論 注射用血塞通（凍干）具有良好的抑制下腔靜脈血栓作用，且無明顯出血副作用，作用機(jī)制與增強(qiáng)纖溶活性、抑制血小板聚集和血管收縮有關(guān)。

Objective To study the inhibitory effect of Xuesaitong Injection (freeze-drying) on inferior vena cava thrombosis in rabbits, observe its bleeding side effect and its effect on coagulation function, and explore its mechanism. Methods Inferior vena cava thrombosis model in rabbits was created by electrical stimulation and stenosis. One hour later, Xuesaitong Injection (freeze-drying) of 5, 10, and 20 mg/kg were injected into ear vein of rabbits for 5 d after surgery, and low molecular weight heparin sodium injection of 100 U/kg was sc given as a positive drug. Bleeding time and blood loss were measured 1 h after infusion. Blood was taken from the jugular vein and prepared into plasma. Activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), and fibrinogen (FIa) were determined by kits, and coagulation factor FIIa, FXa, FXIa, antithrombin III (ATIII), coagulation factor TAT, activated protein C (APC), tissue fibrinolytic enzyme activator (t-PA), plasminogen (PLG), prourokinase (PROUK), trypsin, 6-keto-PGF1a, and thromboxane B₂(TXB₂) were determined by enzyme immunoassay kit. Thrombus was taken out, and wet weight, dry weight and base quality of thrombus were determined. In the platelet aggregation experiment, platelet-poor and platelet-rich plasma were prepared, and collagen (CG), ADP, and arachidonic acid (AA) were used as the inducers. Effects of different concentration of Xuesaitong Injection (freeze-drying) on platelet aggregation were measured using nephelometry. Results Wet weight, dry weight, and base quality of thrombus were all significantly reduced in a dose-dependent manner after injection of Xuesaitong Injection (freeze-drying), which indicated that Xuesaitong Injection (freeze-drying) had a significant inhibitory effect on deep vein thrombosis. After administration of Xuesaitong Injection(freeze-drying), bleeding time and blood loss of rabbits were no significant extended, and APTT, PT, and TT did not change significantly compared with the model group, which indicated that Xuesaitong Injection (freeze-drying) showed no significant bleeding side effects when it played antithrombotic effect. After administration of Xuesaitong Injection (freeze-drying), t-PA and 6-keto-PGF1a in rabbit plasma were significantly increased, and TXB₂ was significantly reduced. In the platelet aggregation experiment, Xuesaitong Injection (freeze-drying) could inhibit platelet aggregation induced by AA in a dose dependent manner. Conclusion Xuesaitong Injection (freeze-drying) has a good inhibitory effect on inferior vena cava thrombosis, and its mechanism of action may be related to enhancing fibrinolysis and inhibition of platelet aggregation and vasoconstriction.

天津市應(yīng)用基礎(chǔ)與前沿技術(shù)研究計劃青年項目（14JCQNJC13300）