目的 觀察山茱萸總萜對KKay糖尿病小鼠的治療作用。方法 選擇KKay糖尿病小鼠模型，分別以低、中、高劑量（0.05、0.10、0.20 g/kg）的山茱萸總萜ig給藥5周。每天給藥前觀察動物一般狀況；給藥前及給藥期間每周測定1次小鼠體質量、空腹血糖；給藥第5周，進行ip葡萄糖耐量試驗（IPGTT），采血測定胰島素（Ins）、糖化血紅蛋白（HbA1c）、糖化血清蛋白（GSP）、總膽固醇（TC）、三酰甘油（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）水平，計算胰島素抵抗指數(shù)（IR）。結果 與模型組比較，山茱萸總萜可以劑量相關性地減輕KKay小鼠體質量，高劑量組第1、2和5周差異顯著（P<0.05）；各劑量組自給藥第1周起即可顯著降低空腹血糖水平（P<0.05、0.01）；高劑量組顯著降低IPGTT中小鼠血糖-時間曲線下面積（P<0.05）；山茱萸總萜可以劑量相關性地降低Ins、HbA1c、GSP、TC、TG、LDL水平、降低IR。結論 山茱萸總萜對于KKay小鼠糖尿病相關癥狀和指標具有劑量相關性的改善作用，可改善脂質代謝紊亂，提示其在治療伴有胰島素抵抗和脂質代謝紊亂的輕、中度2型糖尿病方面具有良好的應用前景。

Objective To investigate effects of terpenes from Cornus Fructus (TCF) on KKay diabetic mice. Methods KKay diabetic mice were ig administered with different doses (0.05, 0.10, and 0.20 g/kg) of TCF for 5 weeks. Animal general condition was observed before dosing every day. Body weight and fasting blood glucose were measured once per week before and during administration. In the fifth week, ip glucose tolerance test (IPGTT) was employed, and levels of insulin (Ins), glycosylated hemoglobin (HbA1c), glycosylated serum protein (GSP), total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), and low density lipoprotein (LDL) in blood were determined. The insulin resistance index (IR) was calculated. Results Compared with model group, TCF dose-dependently reduced body weight and the high dose group was significantly different in first, second and fifth weeks (P<0.05). The fasting blood glucose level (P<0.05, 0.01) was significantly lower since the first week of each dose group. High dose of TCF significantly reduced the area under the curve of blood glucose versus time profile (P<0.05). TCF can dose dependently decreased the levels of Ins, HbA1c, GSP, TC, TG, LDL, and IR. Conclusion TCF can obviously improve the hyperglycemia and insulin resistance in KKay mice. In addition, TCF could improve the disorder of lipids metabolism. Based on these results, we conclude that TCF is a potential candidate for clinical treatment in mild-moderate type 2 diabetes mellitus with insulin resistance and lipid metabolism disorder.

國家自然科學基金青年項目（81403154）