[關(guān)鍵詞]
[摘要]
目的 觀察補腎調(diào)沖方對卵巢早衰模型大鼠血清促卵泡刺激素（FSH）、促黃體生成素（LH）、抗繆勒管激素（AMH）水平及卵巢組織腫瘤壞死因子-α（TNF-α）、干擾素-γ（IFN-γ）蛋白表達(dá)的影響。方法 60只SD雌性大鼠隨機分為6組：對照組，模型組，補腎調(diào)沖方低、中、高劑量（以生藥計24、12、6 g/kg）組，結(jié)合雌激素片（陽性藥）組，每組10只。除對照組外，其余各組均ig雷公藤多苷片造模14 d，造模結(jié)束24 h后，給予相應(yīng)藥物治療36 d。給藥結(jié)束后，酶聯(lián)免疫吸附（Elisa）法檢測大鼠血清FSH、LH、AMH水平，免疫組化法檢測卵巢組織TNF-α、IFN-γ蛋白表達(dá)。結(jié)果 與對照組比較，模型組血清FSH、LH水平、卵巢TNF-α、IFN-γ蛋白表達(dá)均顯著升高，AMH水平顯著降低（P<0.05）；與模型組比較，各給藥組FSH、LH水平、卵巢TNF-α、IFN-γ蛋白表達(dá)均顯著降低，AMH水平顯著升高（P<0.01）。結(jié)論 補腎調(diào)沖方可提高卵巢早衰大鼠卵巢儲備能力，抑制卵泡封閉及黃體細(xì)胞凋亡，對卵巢早衰可能有治療作用。
[Key word]
[Abstract]
Objective To observe the effect of Bushen Tiaochong Prescription (BTP) on sex hormone level and TNF-α, IFN-γ protein expression in rats with premature ovarian failure. Methods Totally 60 SD female rats were randomly divided into six groups with 10 rats in each group:control group, model group, BTP low, medium, and high dose (24, 12 and 6 g/kg in crude drugs) groups, and Conjugated Estrogens Tablets (positive drug) group. Rats were ig administered with tripterygium glycosides for 14 d to make model of premature ovarian failure except for control group. Rats were treated with corresponding medicines for 36 d 24 h after that. Then the enzyme linked immunosorbent assay (Elisa) method was used to detect the levels of AMH, LH, and FSH in serum, and the expression of TNF-α and IFN-γ in ovarian tissue was detected by immunohistochemistry. Results Compared with control group, the levels of serum FSH, LH and ovarian TNF-α, IFN-γ protein expression of model group was increased significantly, and the level of serum AMH was significantly decreased (P<0.05); Compared with model group, levels of FSH, LH, TNF-α, and IFN-γ of BTP groups and Conjugated Estrogens Tablets group were significantly decreased, AMH level was significantly increased (P<0.01) Conclusion BTP can improve the ovarian reserve, inhibit follicle blocking and apoptosis of corpus lutein cells of the premature ovarian rats, which suggests that BTP may have a therapeutic effect on premature ovarian disease.
[中圖分類號]
[基金項目]
河北省省級重大醫(yī)學(xué)科研項目（zyzd2013018）；河北省政府資助臨床醫(yī)學(xué)優(yōu)秀人才培養(yǎng)項目（2014571024）；國家中醫(yī)藥管理局高慧全國名老中醫(yī)藥專家傳承工作室建設(shè)項目（2015048157）
