目的 探討?zhàn)B血祛風(fēng)方對抽動障礙（TD）大鼠紋狀體神經(jīng)元超微結(jié)構(gòu)的影響。方法 采用ig亞氨基二丙腈（IDPN）制備TD大鼠模型，造模成功后將大鼠隨機(jī)分為模型組，養(yǎng)血祛風(fēng)方高、中、低劑量（11.75、23.50、35.25 g/kg）組，硫必利（陽性藥）組，同時(shí)設(shè)立對照組，連續(xù)給藥8周。于給藥第1、4、8周進(jìn)行刻板運(yùn)動評分；末次給藥24 h后，大鼠開顱取紋狀體，多聚甲醛固定后切片、染色，通過透射電鏡觀察紋狀體腹側(cè)部超微結(jié)構(gòu)。結(jié)果 與模型組比較，低、中、高劑量養(yǎng)血祛風(fēng)方和硫必利均對大鼠刻板運(yùn)動評分發(fā)揮顯著改善作用（P<0.05）；養(yǎng)血祛風(fēng)方高劑量組和硫必利組大鼠紋狀體中的神經(jīng)元細(xì)胞、線粒體、軸突、樹突及微血管結(jié)構(gòu)與模型組比較，具有顯著差異。結(jié)論 養(yǎng)血祛風(fēng)方對TD模型大鼠腦細(xì)胞有保護(hù)和修復(fù)作用。

Objective To explore the effect of Blood-nourishing and Wind-expelling Formulae (BWF) on ultrastructure of striatal neurons in tic disorder (TD) rats. Methods TD model was induced by ig injection of iminodipropionitrile (IDPN), then TD rats were divided into model group; BWF low, medium, and high dose (11.75, 23.50 and 35.25 g/kg) groups; and tiapride (positive drug) group by random and meanwhile establish control group. Then the rats were treated for 8 weeks and the stereotyped movement scores were recorded in the first, fourth and eighth week. Operate rats craniotomy for their striatum 24 h after treatment, and then fix, slice, and stain striatum, observe the ultrastructure by transmission electron microscope. Results Compared with model group, low, medium, and high dose of BWF and tiapride significantly improved stereotyped movement scores of rats (P<0.05). TD rats striatum of high dose BWF and tiapride in neuronal cells, mitochondria, axons, dendrites, and microvasculature were significantly different compared with model group. Conclusion BWF plays a role of protection and repairing in TD rats brain cells, which should be paid great attention in the treatment of TD therapy.

北京市自然科學(xué)基金資助項(xiàng)目（7122096）