目的 建立靶細胞萃取延胡索Corydalis Rhizoma中的藥效成分并采用HPLC法進行定量分析的方法。方法 選用人臍靜脈內(nèi)皮細胞（HUVEC）作為靶細胞，萃取延胡索中的藥效成分。在細胞對數(shù)生長期加入延胡索凍干粉，孵育2 h收集細胞并用液氮裂解，得到萃取藥物后的細胞樣品。采用UPLC/Q-TOF-MS對其進行檢測，分析萃取進入細胞內(nèi)的物質(zhì)，即延胡索中進入細胞進一步發(fā)揮藥效的有效成分。最后，用HPLC法對進入細胞中的有效成分進行定量分析。結(jié)果 HUVEC萃取到5個藥效成分，分別為延胡索乙素、四氫小檗堿、延胡索甲素、鹽酸小檗堿、去氫延胡索甲素。同時建立5個成分的含量測定方法。結(jié)論 靶細胞萃取的分析方法可以通過化合物與細胞之間的相互作用，反映出藥物中發(fā)揮藥效的活性物質(zhì)，在中藥復雜體系的研究中起到重要的作用。

Objective To establish a method that using target cell to extract the pharmacodynamic components and quantitative analysis in Corydalis Rhizoma. Methods Take the human umbilical vein endothelial cells (HUVEC) as target cells, and add the freeze-dried powder of yanhusuo in logarithmic growth period. After 2 h incubation, the cells were collected and liquid nitrogen was used to cleave those cell samples. While compounds in the extract of cell samples were determined by UPLC/Q-TOF-MS, the effect of effective components that entered the cell of Corydalis Rhizoma was ananlyzed. Finally, HPLC was used to quantitatively analyze the effective components. Results After HUVEC extraction, five active ingredients were obtained, such as tetrahydropalmatine, berberine, tetrahydroberberine, berberine hydrochloride, and dehydrocorydalinecorydaline, respectively. At the same time, the method was established for the determination of the five components. Conclusion The analysis method of the target cell extraction can reflect the active substance in the medicine, through the interaction between the compound and the cell. This method will play an important role in the study of complex system of traditional Chinese medicine.

國家科技支撐計劃課題（2011BAI07B00）