目的 建立雷公藤多苷片的高效液相指紋圖譜，考察不同生產(chǎn)廠家的雷公藤多苷片的一致性，為更好的完善該制劑的質(zhì)量評價奠定基礎(chǔ)。方法 采用高效液相色譜法，Agilent C₁₈色譜柱（250 mm×4.6 mm，5 μm），流動相為乙腈-水，梯度洗脫（0~70 min，35∶65→100∶0）；體積流量1.0 mL/min；柱溫30℃；檢測波長218 nm；進樣量20 μL。結(jié)果 測定了7個廠家的指紋圖譜，色譜圖差距較大，提示不同廠家的雷公藤多苷片的化學(xué)成分不同或同成分但含量差別較大。確定了22個峰為雷公藤多苷片的共有峰，并利用主成分分析法（PCA）對指紋圖譜進行處理，不同廠家的雷公藤多苷片可明顯區(qū)分。結(jié)論 市售雷公藤多苷片的質(zhì)量參差不齊，不同生產(chǎn)廠家雷公藤多苷片HPLC指紋圖譜差異較大，該方法為更好地控制雷公藤多苷片的內(nèi)在質(zhì)量提供了有益的參考。

Objective To establish an HPLC fingerprint method of Tripterygium Glycosides Tablets (TGT), and to apply this method to determining the consistency of TGT fromdifferent manufacturers and supporting the foundation of quality control. Methods An HPLC analysis was performed on C₁₈ column with water-acetonitrile gradient elution, flow-rate of 1.0 mL/min, column temperature of 30℃, detecting wavelength of 218 nm. Results HPLC fingerprints were obviously different among the seven manufacturers, which indicated that there were different chemical compositions or the same composition with different contents. Twenty-two common peaks were named as characteristic fingerprint peaks. Significant differences were observed among different manufacturers in the principal components analysis score plot. Conclusion Significant differences of quality in tripterygium glycosides tablets on the market are observed. The HPLC fingerprint of TGT is established which provides useful reference for quality control.