目的 探討白藜蘆醇（Rsv）對(duì)咪喹莫特（IMQ）誘導(dǎo)的銀屑病樣皮膚損傷的改善作用及其作用機(jī)制。方法 BALB/c小鼠隨機(jī)分為4組：對(duì)照組、Rsv組、模型（IMQ）組、Rsv治療（IMQ+Rsv）組，每組6只；涂抹IMQ誘導(dǎo)BALB/c鼠耳部銀屑病樣皮膚損傷模型，連續(xù)8 d，同時(shí)涂抹20 mg Rsv予以治療；觀察紅斑及銀屑的多少、游標(biāo)卡尺測(cè)量耳厚度；HE染色觀察組織改變；實(shí)時(shí)熒光定量（qRT-PCR）檢測(cè)組織角蛋白17（K17）、IL-17A、IL-19及IL-23 mRNA表達(dá)；Western blotting檢測(cè)K17蛋白表達(dá)。結(jié)果 與模型組比較，Rsv治療組小鼠紅斑面積明顯減少，表皮鱗屑減少，耳緣厚度顯著降低；HE染色結(jié)果表明，Rsv治療組耳緣組織表皮厚度明顯減輕，炎細(xì)胞浸潤(rùn)減少；與對(duì)照組比較，模型組K17、IL-17A、IL-19及IL-23 mRNA水平、K17蛋白表達(dá)明顯升高，Rsv治療后K17、IL-17A、IL-19 mRNA水平、K17蛋白表達(dá)顯著降低。結(jié)論 白藜蘆醇通過(guò)抑制K17及炎癥分子表達(dá)，改善IMQ誘導(dǎo)的銀屑病樣皮膚損傷。

Objective To observe the therapeutic effect of resveratrol (Rsv) on psoriasis like skin damage induced by imiquimod (IMQ) and study its mechanism. Methods BALB/c mice were randomly divided into four groups:control group, Rsv group, model (IMQ) group and Rsv treatment (IMQ+Rsv) group with six mice in each group. Psoriasis like skin model induced by smearing IMQ in the mice's ear for 8 d, 20 mg Rsv cream were gave at the same time for treatment. Observe erythema and psoriasis degree, and measure ear thickness with vernier caliper. HE staining was used to observe tissue change, real time quantitative PCR (qRT-PCR) was used to detect K17, IL-17A, IL-19 and IL-23 mRNA and Western blotting were used to detected K17 protein expression in tissues. Results Compared with the model group, mice erythema area and the thickness of epidermal scale decreased significantly in Rsv treatment group; HE staining results showed that, Rsv significantly reduced ear marginal tissue epidermal thickness, and diminished severity of the psoriasis-like skin inflammation. qRT-PCR and Western blotting confirmed a Rsv dependent decrease of in K17, IL-17A and IL-19 in mRNA level and protein levels of K17. Conclusion Rsv improves psoriasis like skin damage by down-regulating expression of Keratin17.

陜西省自然科學(xué)基礎(chǔ)研究面上項(xiàng)目（2016JM8046，2014JQ2-8055，2016JM8047）；陜西省衛(wèi)計(jì)委重點(diǎn)項(xiàng)目（2014A）；西安醫(yī)學(xué)院校級(jí)重點(diǎn)建設(shè)學(xué)科資助項(xiàng)目（2015097）