目的 研究姜黃素與甘草次酸單獨(dú)、聯(lián)合用藥對(duì)肝癌細(xì)胞HepG-2增殖的抑制作用，探討姜黃與甘草配伍的合理性和科學(xué)性。方法 不同濃度姜黃素（10.00、5.00、2.50、1.25 μg/mL）、甘草次酸（20.0、10.0、5.0、2.5 μg/mL）及兩者對(duì)應(yīng)濃度聯(lián)合應(yīng)用處理肝癌HepG-2細(xì)胞不同時(shí)間后（8、16、24 h），采用CCK-8法檢測(cè)細(xì)胞增殖抑制率；姜黃素5 μg/mL、甘草次酸10 μg/mL及兩者聯(lián)合應(yīng)用處理HepG-2細(xì)胞24 h后，用流式細(xì)胞儀檢測(cè)細(xì)胞凋亡及細(xì)胞周期變化情況。結(jié)果 姜黃素、甘草次酸及聯(lián)合用藥對(duì)HepG-2細(xì)胞增殖均具有抑制作用，呈劑量相關(guān)性和時(shí)間相關(guān)性，且兩藥聯(lián)合呈現(xiàn)相加或增強(qiáng)的作用；姜黃素、甘草次酸均能誘導(dǎo)HepG-2細(xì)胞凋亡，聯(lián)合用藥后誘導(dǎo)凋亡作用更強(qiáng)；姜黃素、甘草次酸及聯(lián)合用藥對(duì)HepG-2細(xì)胞均具有G2期阻滯作用。結(jié)論 聯(lián)合用藥具有比單用姜黃素或甘草次酸更強(qiáng)的抑制HepG-2細(xì)胞增殖、誘導(dǎo)細(xì)胞凋亡作用，姜黃與甘草配伍具有合理性和科學(xué)性。

Objective To study the inhibitory effect of Curcumin combined with Glycyrrhetinic acid on proliferation of HepG-2 cell, and probe the reasonability and scientificity of curcuma combined with glycyrrhiza. Methods The CCK-8 method was used to test the proliferation inhibition rate of HepG-2 cells after treated with Curcumin (10.00, 5.00, 2.50, and 1.25 μg/mL), Glycyrrhetinic acid (20.0, 10.0, 5.0, and 2.5 μg/mL) and Curcumin combined with Glycyrrhetinic acid in corresponding concentration for different time (8, 16, or 24 h). Flow cytometry was used to detect the cell apoptosis and the cell cycle of HepG-2 cells after treated with Curcumin (5 μg/mL), Glycyrrhetinic acid (10 μg/mL) and Curcumin combined with Glycyrrhetinic acid in corresponding concentration for 24 h. Results Curcumin, Glycyrrhetinic acid and drug combination obviously inhibited the proliferation of HepG-2 cells in a time-and concentration-dependent manner, the effects of combination group was more stronger and showed additive effect or synergistic effect. The apoptosis rate of HepG-2 cells was significantly increased after treated with three groups of drug, combination group showed additive effect; Curcumin, Glycyrrhetinic acid and the drug combination showed significant G2 arrest. Conclusion Curcumin combined with Glycyrrhetinic acid has positive effect on inhabiting the proliferation and promoting apoptosis of HepG-2 cell. Curcuma combined with glycyrrhiza possess reasonability and scientificity.

國家自然科學(xué)基金資助項(xiàng)目（81274147）