目的 建立核磁共振氫譜法測定槲皮素原料藥的絕對含量。方法 以DMSO-d6 為溶劑，馬來酸為內(nèi)標，采集槲皮素和馬來酸混合物的核磁共振氫譜。以槲皮素中化學位移在δ7.50~7.58和馬來酸化學位移在δ6.26的質(zhì)子信號峰為定量峰，測定槲皮素的量，并與質(zhì)量平衡法比較。結果 槲皮素與馬來酸峰的面積比（A_s/A_r）與其質(zhì)量比（m_s/m_r）的線性回歸方程為y=2.963 x+0.134 1（r=0.999 3）。測得3批槲皮素的質(zhì)量分數(shù)分別為85.20%、84.93%和85.27%，平均質(zhì)量分數(shù)為85.13%，RSD為0.21%。測定結果與質(zhì)量平衡法定值基本一致。結論 所建立的氫核磁共振定量法準確、快速，為質(zhì)量平衡法提供有力佐證。

Objective To establish a novel method to determine the absolute content of quercetin by proton nuclear magnetic resonance (qNMR). Methods DMSO-d₆ was employed as solvent, and maleic acid as an internal standard. Proton signal peaks at δ7.50-7.58 and δ6.26 of maleic acid were served as quantification peaks. The content of quercetin is determined with qNMR in comparison with the results obtained by mass balance method. Results Linear regression of quantitative peak areas ratio (As/Ar) of quercetin-maleic acid vs mass ratio (m_s/m_r) yielded a correlation coefficient of 0.999 3 and a regression equation of y=2.963 x+0.134 1. The contents of three batches quercetin were 85.20%, 84.93%, and 85.27%,the average was 85.13% and its RSD was 0.21%. The results were generally consistent with that of mass balance methods. Conclusion This method was easy and simple to handle, and the analysis results were accurate. It could be the complementary for the mass balance method.