目的 研究利尿合劑的抗炎鎮(zhèn)痛及體外抑菌作用，為臨床用藥提供科學(xué)的理論依據(jù)。方法 采用二甲苯致耳腫脹實(shí)驗(yàn)、冰醋酸致小鼠扭體反應(yīng)實(shí)驗(yàn)、冰醋酸致小鼠腹腔毛細(xì)血管通透性增加實(shí)驗(yàn)及福爾馬林致痛實(shí)驗(yàn)，觀察利尿合劑高、中、低劑量組（以生藥計(jì)18.0、9.0、4.5 g/kg）的抗炎鎮(zhèn)痛作用；采用K-B紙片擴(kuò)散法研究利尿合劑對(duì)金黃色葡萄球菌和大腸桿菌的體外抑菌作用。結(jié)果 利尿合劑高劑量組可明顯減輕小鼠耳廓腫脹度（P<0.01），高、中、低劑量明顯減少冰醋酸致小鼠扭體反應(yīng)次數(shù)（P<0.01），明顯抑制冰醋酸致小鼠腹腔毛細(xì)血管通透性增加（P<0.05、0.01），顯著降低福爾馬林致痛實(shí)驗(yàn)的第I時(shí)相的疼痛強(qiáng)度（P<0.01），對(duì)第Ⅱ時(shí)相的疼痛強(qiáng)度沒(méi)有明顯影響；利尿合劑對(duì)金黃色葡萄球菌和大腸桿菌的抑菌率分別為95.04%、37.44%。結(jié)論 利尿合劑具有較好的抗炎鎮(zhèn)痛作用，對(duì)金黃色葡萄球菌和大腸桿菌具有體外抑菌作用。

Objective To investigate the analgesic and anti-inflammatory effects of diuretic mixture and its bacteriostasis effect in vitro, and to provide scientific basis for clinical application.Methods Ear swelling test induced by xylene, twisting reaction test induced by acetic acid, capillary permeability increase in abdominal cavity of mice induced by acetic acid, and pain test induced by formalin were used to observe the analgesic and anti-inflammatory effects of diuretic mixture at high, middle and low doses (crude drug 18.0, 9.0, and 4.5 g/kg). Bacteriostatic activities of diuretic mixture were tested by K-B paper disc diffusion method.Results Diuretic mixture alleviated ear edema in mouse model at high dose (P<0.01). Diuretic mixture at high, middle, and low dose could effectively decrease the twisting reaction (P<0.01), inhibit capillary permeability (P<0.05 or 0.01), and ease the ache degree of mice induced by formalin in the first phase (P<0.01), but there was no significant difference in the degree of pain intensity of phase II. The inhibitory rates of diuretic mixture on Staphylococcus aureus and Escherichia coli were 95.04% and 37.44%, respectively.Conclusion Diuretic mixture has significant effects on analgesia and anti-inflammation and against S. aureus and E. coli in vitro.

中山市科技計(jì)劃項(xiàng)目（20132A166，2015B1049）